02 May WHAT IS THE VALUE (IN VOLTS) OF THE STANDARD REDUCTION POTENTIAL FOR THIS HALF-REACTION?
Please use the attached table for values. Thanks! A. Write a complete balanced redox reaction, which would occur spontaneously in the forward direction, where electron(s) are transferred between cytochrome a3 and NADP. Be sure to accurately indicate the chemical and/or ionic states of all species on both the reactant and product sides, and also indicate appropriate stoichiometries. B. For the forward reaction which species is acting as the oxidizing agent and which is the reducing agent? Be specific (indicate the accurate chemical form). C. Write the half-reaction involving cytochrome a3. For the overall redox reaction youve written (Part A), would this half-reaction occur as an oxidation or a reduction? What is the value (in volts) of the standard reduction potential for this half-reaction? D. Write the half-reaction involving NADP. For the overall redox reaction youve written (Part A), would this half-reaction occur as an oxidation or a reduction? What is the value (in volts) of the standard reduction potential for this half-reaction? E. Calculate ΔE° for the overall redox reaction (from Part A). Show all work & indicate proper units. F. Does your calculated ΔE° value (Part E) support your contention that the overall redox reaction (Part A) would be spontaneous in the forward direction? Explain your answer in terms of the relative electron affinities of the species involved. G. Calculate ΔG° for the overall redox reaction. Show all work & indicate proper units. Under standard biochemical conditions, is this reaction endergonic or exergonic? Explain. H. Calculate Keq for the overall redox reaction. Assume 25°C. Show all work & indicate proper units. I. In a broader cellular context, what is the typical role of the universal electron carrier NADP? Is this carrier more likely to be involved in anabolic or catabolic reactions/processes? Is this carrier more likely to act as an electron donor or an electron acceptor? Explain your answers. J. Based on your answer to Part I, would you predict the actual free energy change, ΔG, of the overall redox reaction (from Part A), to be negative, close to zero, or positive? In other words, do you think this redox reaction under typical cellular conditions would still be spontaneous in the forward direction? Why or why not? Empirically, how do you think the values of ΔG° and ΔG would compare with one another? Carefully & completely explain all of your answers; you should include an explicit discussion of NADP as it relates to part I. K. Suppose you are told that the actual change in reduction potential, ΔE, is -0.06 volts. Calculate ΔG (show all work & indicate proper units). Do these ΔE and ΔG values make sense in terms of your answer to Part H and I? If so, support your contention. If not, what might be a possible explanation?
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