02 May DESCRIBE WHAT IS KNOWN ABOUT THE CELLULAR AND PHYSIOLOGICAL PROCESSES THAT THE PROTEIN/GENE IMPACTS AND HOW IT HAS THE EFFECT.
Biochemistry Term-Paper Requirement and Format
General Topics:
Choose an enzyme or protein to describe and discuss its biochemical and molecular properties, metabolic function, physiological roles, and potential applications in either (A) human/animal nutrition, health, or disease, or (B) plant production/agricultural applications, such as improvements of yield, resistance to drought or disease, human/animal nutrition, or industrial-use quality.
The Paper must include the following elements:
1. Show the complete DNA sequence of the gene that encodes the protein of your choice. Include the database (i.e. website) from which you obtain the sequence and reference. Use Genbank and NCBI Molecular Biology Resources to search gene/protein sequence (e.g. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed). Describe briefly, if applicable, the gene family, using in a specific organism as an example, such as how many such genes in mouse.
2. Mark the translation start codon and stop codon of the gene; show the deduced amino acid sequence encoded by the gene; describe what is known about posttranslational and/or cotranslational modifications of the protein. Cite the reference from which you get the information.
3. Describe the enzymatic reaction and metabolic function of the protein. Include the specific reaction catalyzed by the enzyme and the metabolic pathway/network that this enzyme is involved in.
4. Describe how the enzyme/protein is regulated, such as long-term, short-term regulation, cofactors, etc.
5. Describe what is known about the cellular and physiological processes that the protein/gene impacts and how it has the effect. Cite the reference from which you get the information.
6. Evaluate critically the gaps of the current knowledge about the regulation and function of the enzyme/protein; what more needs to be learned for a better understanding of the protein and its functions for potential applications.
7. Discuss the potential implication and applications of the above information in/to human nutrition, health, or disease, or in plant growth/agricultural application, such as better yield, more resistance to drought or disease, better nutritional value, or better industrial-use quality.
8. Propose at least one experiment/study that you may use to advance the understanding of the enzyme/protein or test the application of the knowledge for improvements as outlined in 7.
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