09 Jun SLE111 Bioinformatics Assignment Gene Identification
Question
Bioinformatics Assignment SLE111, Bioinformatics Assignment Gene Identification, an Introduction to Bioinformatics and Databases Assessment This assignment is marked out of 70, and is worth 7% of your final mark for the unit. Read this entire document, including the supplementary information at the end, before commencing the assignment. Introduction/Background: You are a member of a laboratory that has sequenced DNA from a gene fragment derived from a mixed sample of pond organisms. It is your job to find out the meaning behind the unknown DNA sequence. Your supervisor has asked you to analyse the sequence and answer some related questions. Learning Outcomes (Aims): 1. To gain experience in using the National Centre for Biotechnology Information website, which gives access to numerous nucleic acid and protein databases, and much more. 2. To identify the gene that an unknown sequence of DNA (the gene fragment) is part of. 3. To appreciate the differences between nucleotide sequence (e.g., of a gene) and amino acid sequence (e.g., of a protein), and discern properties of such sequences. 4. To comprehend and synthesise information from scientific papers and cite these papers in the proper format. The website you will need to familiarize yourself with in order to complete this assignment is: NCBI: www.ncbi.nlm.nih.govand, within that site, you will be usingNCBI BLAST: http://blast.ncbi.nlm.nih.gov/BLAST stands for Basic Local Alignment Search Tool. It is a program that compares a query sequence of nucleotides or amino acids (depending on which type of BLAST that is used) against all the nucleotide and amino acid sequences that have been lodged in genetic databases worldwide. The ‘alignment’ refers to how the comparisons are made: that is, by aligning your query sequence against all sequences in the database and looking for the best match. A BLAST search is the standard way for biologists to identify sequences of genes or proteins, or their closest…
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