09 Jun BIO Lab 20 Pre-Lab Questions
Question
Experiment 1: Paper Chromatography
In this experiment, you will separate plant pigments using chromatography. You will also measure the rate of photosynthesis in isolated chloroplasts using the reduction of the dye 2,6-Dichloroindophenol (DPIP) as the measurement tool. The transfer of electrons during photosynthesis reduces DPIP, changing it from blue to clear.
Materials
10 mL 4.5% Acetic Acid (Vinegar), C2H4O2
10 mL Acetone (Nail Polish Remover), C3H6O
30 cm Aluminum Foil
(4) 100 mL Beakers
(1) 250 mL Beaker
20 cm Cheesecloth
3 mL 1% 2,6-Dichloroindophenol, DPIP
(1) 12 x 12 cm Chromatography Paper Piece
(1) 100 mL Graduated Cylinder
10 mL Mineral Oil
3 mL 0.1 M Phosphate Buffer, P
8 Pipettes
(1) Resealable Plastic Bag
Rubber Band (Large; contain latex, handle with gloves on if allergic)
Ruler
Wooden Stir Stick
100 mL 0.5 M Sucrose Solution (cold!), C12H22O11
3 Test Tubes (Glass)
Test Tube Rack
*Cutting Board
*Kitchen Knife
*Light Source
*Pencil
*Quarter
*Scissors
**Spinach Leaves (Fresh)
*Tape (masking or Scotch®)
*Water
*You Must Provide
*Keep these leaves if performing Experiment 2 within a few days of Experiment 1.
Pre-Lab Questions
1. Describe how the functional units for beta carotene, xanthophyll, chlorophyll A, and chlorophyll B are different. Be sure to identify the subunits that adhere to paper during chromatography.
2. Describe a technique for measuring photosynthetic rate.
3. Many deciduous trees have leaves which turn yellow in the fall. What do you suppose is happening in the leaves at the cellular and molecular level?
4. Chloroplasts and mitochondria are both are unusual in that they have double membranes and contain their own set of DNA. Can you think of any explanations for this observation?
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