10 Jun BIOL 160 – Which of the following was NOT an ingredient
Question
Which of the following was NOT an ingredient in our PCR master mix:
A. DNA nucleotides
B. a heat-stable DNA polymerase
C. primers
D. RNA
E. DNA
During a PCR reaction, what is meant by the “Annealing” phase?
A. DNA strands separate- Hydrogen bonds are being disrupted
B. Full length DNA strands are forming hydrogen bonds, brining the strands together
C. primers are forming hydrogen bonds with complementary DNA sequence
D. polymerase is polymerizing new DNA stands
Short Tandem Repeats (STRs) are
short repeated DNA sequences within introns (non-coding) regions of DNA
present in many different chromosomal locations
highly variable with the human population (the number of repeats is variable)
of unknown function
all of the above
What is the MAIN purpose of running the PCR samples on an agarose gel using electrophoresis?
to separate the DNA products by their length (in base pairs)
to make more copies of the DNA
to purify the PCR-DNA product away from the other master mix components
to learn the exact sequence of the DNA samples
When first setting up your gel electrophoresis, the DNA samples will be added to “wells” made in the gel. These wells are closest to the
A. positive electrode
B. negative electrode
C. neutral electrode
Most of our PCR reactions will contain 2 DNA products. What is the best explanation of this?
the various suspects are heterozygous for STR number at this region (locus)
the various suspects are homozygous for STR number at this region (locus)
the PCR worked TWICE as well as usual and we got double the DNA, because we are awesome
all of the above
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