10 Jun BIOL 303 – Which of the following is NOT true for proteins
Question
Which of the following is NOT true for proteins that are synthesized on membrane-bound ribosomes?
A.They may be secreted from the cell.
B.They may traffic to mitochondria or peroxisomes
C.They may traffic to the lysosome
D.When they leave the ER they first go to Golgi.
You use homogenization and subcellular fractionation with differential centrifugation to isolate microsomes of the rough ER. You add a mixture of mRNAs- some that encode proteins with signal sequences and some that encode cytosolic proteins. You allow translation to proceed and analyze the protein content within themicroscomes. What proteins do you expect to find in the microsomes?
A.All of the proteins
B.Only the proteins containing signal sequences
C.Only the cytosolic proteins
D.None of the proteins will be in the microsomes
In the next experiment, you decide to first translate one of the mRNA sequences in to protein containing a signal sequence. Next, you add the microsomal membranes to the test tube. After this step, do you predict that you will be able to recover microsomes with the translated proteins inside?
A.yes
B.no
Which of the following is NOT true about the extracellular portion of an integral membrane protein at the plasma membrane?
A.It may contain disulfide bonds that form in the lumen of the ER.
B.It may contain oligosaccharides added in the ER and Golgi.
C.It is post-translationally modified within the reducing environment of the cytosol
D.All of the above are true
If the carboxyl end of an integral membrane protein is extracellular, then
A.the signal sequence of the protein is not at the amino end of the protein.
B.the amino end of the protein will enter the ER first
C.the signal sequence will not bind to the SRP.
D.the amino end of the protein will be in the lumen of the ER.
?
You are studying an integral membrane protein of the plasma membrane. It has a single transmembrane domain and the N-terminal portion of this protein is extracellular. You decide to perform mutagenesis studies of the stop-transfer sequence, and you mutate all of the hydrophobic residues to hydrophilic residues. What do you predict will happen?
A.The protein will be transported to the plasma membrane and its N-terminal end will be extracellular.
B.The protein will be transported to the plasma membrane and its C-terminal end will be extracellular.
C.The protein will enter the ER lumen and eventually be secreted to the extracellular space
D.The protein will be translated on free ribosomes in the cytosol and transported to the mitochondria.
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