11 Jun BIOL 326 – What is true about gene expression
ark ALL that apply. What is true about gene expression?
a.some genes are only expressed under certain environmental conditions
b.some DNA sequences that have features of a gene are never expressed; those are called ‘pseudogenes’
c.some genes are only expressed in certain tissues
d.two genes, when expressed, may differ in the number of transcript molecules they produce
e.some genes are only expressed during a developmental stage, but not others. For example: the globin paralogs
f.some genes are expressed all the time, in every tissue
2) Which of the following methods is NOT used to study gene expression?
a.DNA microarray
b.RNA-seq
c.Northern blot
d.DNAse I footprint
e.RT-PCR
3) Mark ALL that apply. What do Northern blots and Microarrays have in common?
a.in both, it is necessary to include a control that normalizes the quantity of mRNA loaded
b.the measument of how much a gene is expressed depends on hybridization between probe and transcript
c.they both study a large number of genes
d.they both study gene expression by measuring the accumulation of protein
e.they both study gene expression by measuring the accumulation of mRNA
4) How can microarrays help you find non-coding sequence?
a.non-coding sequence will show up as yellow dots (equal hybridization between treatments)
b.non-coding sequence cannot be used as probes
c.non-coding sequence should not hybridize to any transcript pool
d.non-coding sequence will preferentially hybridize one transcript pool over another
5) Order from less complex to more complex…
a.transcriptome < proteome < genome b.proteome < transcriptome < genome c.transcriptome < genome < proteome d.genome < transcriptome < proteome 6) The following epigenetic modifications control gene expression: Mark all) DNA methylation dominance/recessiveness transposon interrupting a gene histone acetylation histone methylation 7) Regulatory protein myosin (muscle protein) histone actin (forms cytoskeleton) transcription factor 8) Match to correct definition primary structure secondary structure tertiary structure Quaternary structure A.alpha helices, beta sheets B.amino acid sequence C.folded protein D.monomeric protein E.multimeric protein 9) When was the main secondary structure of the zika virus? Use PDB to find out. alpha helix beta sheets turns 10) Mark ALL that apply. You wish to isolate an enzyme, and wish it remains active. Which techniques would most likely preserve enzyme activity? SDS-PAGE 2-D gels affinity chromatography size-exclusion chromatography ion-exchange chromatography mass spectrometry 11) Mass spectrometry a.allows identification of any protein b.allows identification of proteins if the peptides that compose the protein are in the database used to compare against the spectrometry data obtained c.separates full proteins, based on mass and charge d.only provides protein identification, but not quantification 12) Match to correct definition glycosylation phosphorylation ubiquitination acetylation A.promotes or inhibits enzyme activity B.regulates gene expression C.marks proteins for turnover D.adhesion; ligand-binding; cell ID (recognition)
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