11 Jun BIO205, Microbiology Quiz 4
Question
1 of 30
What product of transcription serves as the code for synthesizing new proteins through the process of
translation?
A new strand of DNA
rRNA
tRNA
mRNA
None of the above 2 of 30
An enzyme produced in response to the presence of a substrate is called ________.
an inducible enzyme
a repressible enzyme
a restriction enzyme
an operator
a promoter 3 of 30
Transformation is the transfer of DNA from a donor to a recipient cell ________.
by a bacteriophage
as naked DNA in solution
by cell-to-cell contact
by crossing over
by sexual reproduction 4 of 30
Genetic change in bacteria can be brought about by ________.
mutation
conjugation transduction
transformation
All of the above 5 of 30
Which of the following is not true of a bacterium that is R+?
R+ refers to the possession of a plasmid.
R+ can be transferred to a recipient cell.
It is resistant to certain drugs and heavy metals.
It is F+.
None of the above 6 of 30
According to the operon model, for the synthesis of an inducible enzyme to occur, the ________.
end-product must not be in excess
substrate must bind to the enzyme
substrate must bind to the repressor
repressor must bind to the operator
repressor must not be synthesized 7 of 30
Synthesis of a repressible enzyme is stopped by ________.
the allosteric transition
the substrate binding to the repressor
the corepressor binding to the operator
the corepressor-repressor binding to the operator
the end-product binding to the promoter Figure 8.2 8 of 30
In Figure 8.2, if base 4 is thymine, what is base 4′?
Adenine
Thymine
Cytosine
Guanine
Uracil 9 of 30
In Figure 8.2, if base 4 is thymine, what is base 11′?
Adenine
Thymine
Cytosine
Guanine
Uracil 10 of 30
In Figure 8.2 shows a figure of an unwound segment of DNA being replicated, base 2 is attached to
_________. ribose
phosphate
deoxyribose
thymine
Can’t tell 11 of 30
The reaction catalyzed by reverse transcriptase is ________.
DNA mRNA
mRNA cDNA
mRNA protein
DNA DNA
None 12 of 30
Subunit vaccines can be made by genetic engineering of yeast cells. A side effect of this kind of vaccination
might be ________.
the disease
a yeast infection
due to extraneous material
that the vaccine doesn’t provide immunity
None of the above 13 of 30
E. coli can make insulin because ________.
it needs to regulate its cell-glucose level
it’s an ancient gene that now has no function
the insulin gene was inserted into it
it picked up the insulin gene from another cell
No reason; it doesn’t make insulin. 14 of 30 The value of cDNA in genetic engineering is that ________.
it lacks exons
it lacks introns
it’s really RNA
None of the above 15 of 30
Which enzyme does not make sticky ends?
Enzyme Recognition
BamHI GGATCC
CCTAG?G Enzyme Recognition
EcoRI GAATTC
CTTAA?G Enzyme Recognition
HaeIII GGCC
CC?GG Enzyme Recognition
HindIII AAGCTT
TTCGA?A Enzyme Recognition PstI CTGCAG
G?ACGTC 16 of 30
Which enzyme would cut this strand of DNA:
GCATGGATCCCAATGC?
Enzyme Recognition
BamHI GGATCC
CCTAG?G Enzyme Recognition
EcoRI GAATTC CTTAA?G
Enzyme Recognition
HaeIII GGCC
CC?GG Enzyme Recognition
HindIII AAGCTT
TTCGA?A Enzyme Recognition
PstI CTGCAG
G?ACGTC 17 of 30
An example of gene therapy is ________.
insertion of the insulin gene in E. coli.
insertion of the insulin gene in a mammalian cell culture
insertion of the insulin gene in a diabetic person’s pancreas cells
injection of insulin into a diabetic person
None of the above 18 of 30
Humans use __________ to select desirable breeds of animals or strains of plants to cultivate.
replica plating
mutation
artificial selection
natural selection
All of the above 19 of 30
A restriction fragment is ________.
a gene
a segment of DNA
a segment of RNA
None of the above 20 of 30
Which of the following can be used to make recombinant DNA?
protoplast fusion
Tungsten "bullets"
microinjection
Transformation
All of the above 21 of 30
What do you typically call segments of DNA?
Chromosome
Genome
Genes
Fragments 22 of 30
When DNA becomes mRNA what has happened?
An organism has died
Translation
Transcription
Ligation 23 of 30
What is the purpose of the Ames test?
To test for AIDS
To screen mutagens suspected of causing cancer in humans
To diagnose cancer
To see if DNA has been destroyed 24 of 30
What is typically the final step in genetic engineering?
Plasmid is formed
Vector is selected
DNA is copied
Genes or proteins are harvested 25 of 30
How many properties of vectors are there?
3
2
1
4 26 of 30
What do you call the science of determining what all of the proteins are expressed in a cell?
Genomics
Southern blotting
Proteomics
Orthocloning 27 of 30
Which of the following groups of microorganisms has the rRNA loop present
Archaea
Eukarya
Bacteria
Viruses 28 of 30
Which of the following is a Class? Eukarya
Proteobacteria
Carnivore
Mammalia 29 of 30
Why are the use of gram staining and acid fast staining used in a clinical setting?
They are expensive
They are slow
They are quick and provide a good starting point for testing and information
They conclusively identify your organism 30 of 30
What technique is used to identify bacteria in a sample without culturing the bacteria?
Fatty acid profiling
Flow Cytometry
Phage Typing
Serology
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