11 Jun U. Houston BIOLOGY 1313 – Explain the potential influence
uestion
1)Explain the potential influence of mutations on the quality of the protein-DNA
interactions and the potential impact of these mutations on gene expression.
2)Be able to examine and interpret the genetic organization of a regulatory circuit
and given some specific constraints make sensible predictions about the behavior of
the circuit including the possible influence of environmental or genetic
perturbations (changes).
3)Given information regarding the allosteric regulation of a DNA binding protein, be
able to predict what effect changing the concentration of the allosteric regulator
would have on the binding of a transcription factor to a regulatory element.
4)Be able to rank the different levels of regulation discussed in class by energy
efficiency for the cell.
5)Using a regulatory network, accurately interpret relationships between
genes/proteins in the network and predict the location of regulation by various
products in the pathway.
6)Describe how genomic DNA is package both in prokaryotes and eukaryotes and
the contributions of basic proteins, such as histones and histone-like proteins, to this
packaging.
7)Create and discuss a picture of the different levels of organization in DNA in
prokaryotes and eukaryotes and put each organizational level in the context of
challenges faced during the cell cycle.
8)Identify the signals responsible for entering the different phases of the cell cycle
and what happens if these are disrupted.
9)Explain the sequence of events that need to occur during mitosis and why they are
necessary including the roles of microtubules, motor proteins, centrosomes, and the
level of DNA condensation.
10)Explain the sequence of events that need to occur during meiosis and why they
are necessary, including
the roles of microtubules, motor proteins, centrosomes, and the level of DNA
condensation.
11)Create and discuss a picture that illustrates the importance of crossing over and
chromatid exchange during meiosis I and explain what happens if these cross over
events do not occur.
12) Describe how allelic segregation and independent assortment result in
inheritance of characteristics through the process of meiosis and sexual
reproduction.
13)Describe the basic principles of Mendelian inheritance (segregation and
independent assortment) and apply these principles to the inheritance of nuclear
genes to interpret the results of sexual crosses.
14)Be able to create, use, and analyze a Punnett Square to determine the genotype
or phenotype of an organism.
15)Describe the challenges of applying the principles of Mendelian inheritance to
the inheritance of mitochondrial and plastid genes
16)Compare the similarities and differences between prokaryotic and eukaryotic
transcriptional regulatory components (the parts on the DNA and the proteins
involved) and the mechanisms that are used for regulating transcription in each
case.
17)Define loci, heterozygous, homozygous, and allele.
18) Determine genetic relationships based on allelic inheritance.
19) Distinguish between positive and negative regulation.
20) Identify modes of regulation based on a gene pathway.
21)Design an experiment to determine the cause of a mutant phenotype.
22) List the phases of the cell cycle and related them to cell division.
23) Construct a pedigree and evaluate the inheritance patterns in families based on
phenotypic observations: dominance, recessive, co-dominant, X-linked,
heterozygous, homozygous, genotype and phenotype.
24)Compare and contrast Mitosis and Meiosis: sister chromatid, chromosome,
homologous chromosomes and the phase of cell cycle.
25) Define heterozygous, homozygous, mutant, wild type, dominant, recessive,
allele, gene and chromosomes.
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