11 Jun Colorado BZ 350 – You have found a mutant phenotype
Question
You have found a mutant phenotype in your lab mice that is associated with reduced metabolic rate. You are interested in determining the genetic basis for this phenotype. You have generated inbred (“true-breeding”) lines with both “normal” and “low” metabolic rates. For each of the following crosses, predict what percentage of both male and female offspring will have the low metabolic rate phenotype assuming the gene is autosomal. Then repeat your predictions under the assumption that gene is X-linked. Finally, repeat your predictions under the assumption that the gene is mitochondrial. You can assume the following for this question: 1) a single gene is responsible for the change in phenotype, and 2) the allele for low metabolic rate is recessive (where applicable). [You do not need to explain your answers for this question].
a. Inbred “low” female x inbred “normal” maleb. Inbred “normal” female x inbred “low” malec. F1 female from cross (a) x F1 male from cross (a) d. F1 female from cross (b) x F1 male from cross (b)
Fill out this table with the predicted percentages of offspring with the “low” phenotype.
AutosomalX-linkedMitochondrial
Male Offspring
Female Offspring
Male Offspring
Female Offspring
Male Offspring
Female Offspring
abcd
2. Based on your answers to question #1, which cross would you recommend as the most efficient way to determine the location of the gene in question? [Note that there are multiple reasonable answers to this question. What is most important is that you clearly
explain the logic for why you chose the cross that you did.]
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