12 Jun Highland BS203 – How should you hold the inoculating
Question
How should you hold the inoculating loop? Select one:
a. Balanced on top of the tubeb. Like a pencilc. With dish glovesd. Maintain it at an angle at all timese. In a test tube rack at all times
Question 2
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If you are going to mix a broth culture prior to transfer, what is a safe way to do this without special equipment? Select one:
a. Tap the tube with your fingersb. Vortex itc. Stick the tube in the outer coned. Use a inoculating loop
Question 3
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Beginning at the base of the slant surface and gently moving the loop back and forth as you withdraw is called: Select one:
a. Slant mixingb. Fishtail inoculationc. Zig-zag streakingd. Quadrant streakinge. Squirrel inoculation
Question 4
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The inoculating (wire) loop is best sterilized by: Select one:
a. Passing it through the inner coneb. Placing it in agarc. Passing it through the outer coned. Putting it in a glass tube
Question 5
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Considering the cultures to be used for inoculating each medium in this exercise, how many microbial types should you expect to see in/on each medium? Select one:
a. 2b. 3c. 0d. 1e. Several
Why would you hold a glass tube with liquid broth at an angle during the process of inoculation? Select one: a. To grip the tube better b. To minimize contaimination, such as air-borne microbes c. The protect your eyes d. To minimize spilling
Question 7 Not yet answered Marked out of 1.0Remove flag Question text Which one of the following would you inoculate if you wanted to keep the culture for several weeks in refrigeration? Select one: a. Glass tube b. Agar slant c. Liquid broth d. Vortex
Question 8 Answer saved Marked out of 1.0Flag question Question text Which of the following is common to minimize airborne contamination? Select one: a. Hold open tubes at an angle b. Perform the experiment in a windy room c. Breathing in sample d. Keep a microscope nearby e. Make someone else do the experiment
Question 9 Answer saved Marked out of 1.0Remove flag Question text These are commonly used for growing stock cultures that can be refrigerated for several weeks: Select one: a. Gym socks b. Agar slants c. Petri dishes d. Cotton swabs e. Broth tubes
Question 10 Answer saved Marked out of 1.0Flag question Question text After inoculating the loop through the flame, why does wire need to cool? Select one: a. Can liquify the wire b. Can cause overgrowth of bacteria c. Can kill microbes and cause aerosols of microbes d. Can start a fire e. Can liquify the entire agar in tube
How can a hot sterile flame loop be cooled quickly to use in the next streak? Select one: a. Put in sterile portion of agar plate b. Blow on it c. Stick the loop in ice d. Set the loop on the lab bench e. Pour tap water on the loop
Question 12 Answer saved Marked out of 1.0Flag question Question text What would likely happen if the inoculum was not spread adequately over the agar surface? Select one: a. Contamination b. Degradation c. Sterilization d. Uneven distribution e. Cells will not grow
Question 13 Not yet answered Marked out of 1.0Remove flag Question text An isolated, single colony forms after incubation of a: Select one: a. Virus (viral particle arrangement in some cases) b. Single cell (cell arrangement in some cases) c. Sterile agar plate d. Sterile flame loop e. Liquid mixed culture
Question 14 Not yet answered Marked out of 1.0Flag question Question text In a quadrant streak, why do you intersect the previous lines and not the ends of the streak? Select one: a. More cell amount variability at ends, usually higher cell population b. Keep streaks in the middle of the plate c. Prevent cell death d. To maintain a mixed culture e. To count CFUs
Question 15 Not yet answered Marked out of 1.0Flag question Question text On a typical plate (100 cm^2), you spread 600 cells. What would likely be your observation after a couple days of incubation? Select one: a. Achieved isolated colonies throughout b. Cells did not grow c. Only a few cells grew d. Too many cells for isolated colonies e. Cells will start anti-fermentation
What is the primary purpose of the streak plate method? Select one: a. Quantify CFUs b. Isolate an individual colony c. Quantify the cell count of a mixed culture d. Inoculate a culture on a plate e. Confirm the identity of an unknown genus
Question 17 Not yet answered Marked out of 1.0Flag question Question text Which of the following is used in the spread plate and not the streak plate method? Select one: a. Glass spreading rod b. Flame loop c. Agar plate d. Bunsen burner/sterile utensil e. Starting culture to sample
Question 18 Not yet answered Marked out of 1.0Flag question Question text If you want to isolate a colony from a culture with a high cell density, which of the following would be preferred? Select one: a. Spread plate method b. Zigzag pattern method c. Quadrant streak method d. Triple streak method e. Liquid inoculation
Question 19 Not yet answered Marked out of 1.0Flag question Question text You have a mixed culture of unknown bacteria and want to identify one of the species by several subsequent experiments (needing multiple colonies of a pure culture). You do a quadrant streak and transfer to a sterile liquid medium. What next step makes the most sense? Select one: a. Perform a Gram stain b. Note all colony morphology characteristics c. Use the spread plate method or new streak plate on an isolated colony of the plate d. Quantify the cells e. Repeat using the other two methods of isolation
Question 20 Not yet answered Marked out of 1.0Flag question Question text What is a major difference between the quadrant streak and zigzag inoculation method? Select one: a. Using a sterile instrument for streaking after the initial streak b. Identifying a mixed culture c. Amount of space of plate the experiment uses d. Type of agar that can be used e. Possibility of cutting the agar
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