12 Jun Arizona BIOLOGY 101 – DNA Extraction Gel Electrophoresis
Question
BIO 101
Visit http://learn.genetics.utah.edu/content/labs/ and complete the following virtual labs:
DNA Extraction
Gel Electrophoresis
PCR
DNA Microarray
Lab 1: DNA Extraction: Complete the lab and answer the following questions.
1. (5 points) What is the purpose of the salt in this lab?
2. (5 points) What is the purpose of the isopropyl alcohol in this lab?
3. (25 points) How is this technology applied in the real world? Discuss one application of this
technology. Cite sources as needed. (1 page maximum)
Lab 2: Gel Electrophoresis: Complete the lab and answer the following questions.
1. (5 points) When performing gel electrophoresis, what is the purpose of the “DNA size standard,” and
why is it used?
2. (5 points) Why does the DNA show up when viewed in the UV light box?
3. (25 points) How is this technology applied in the real world? Discuss one application of this
technology. Cite sources as needed. (1 page maximum)
Lab 3: PCR: Complete the lab and answer the following questions.
1. (5 points) What is a primer, and how does it work in PCR?
2. (5 points) After 30 cycles, how many fragments that contain the target sequence will be present? How
many copies of the longer-length molecule will be present?
3. (25 points) How is this technology applied in the real world? Discuss one application of this
technology. Cite sources as needed. (1 page maximum)
Lab 4: DNA Microarray: Complete the lab and answer the following questions.
1. (5 points) According to the lab, how can you tell if a gene is turned on or turned off?
2. (5 points) After completing the microarray, what did the red dots represent?
3. (25 points) How is this technology applied in the real world? Discuss one application of this
technology. Cite sources as needed. (1 page maximum)
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