12 Jun If gene D is found in a three gene operon
Question
1. If gene D is found in a three gene operon (the D, E,F, OPERON), a gene D missense mutation will have a the same impact on a cell compared to if gene D were not found in an operon. Choose answer below that BESTS support your answer.
a. translation of gene D will be identical in both cases
b. genes E & F will be able to compensate for this gene x mutation
c. transcription of gene e and f will be affected as well
d. expression of the entire operon will be blocked.
2. The m RNA splicing reaction is initiated by the branch point A, a specific nucleotide found in the intron. if the branch point A were replaced with a dATP molecule and therefore the splicing reaction would not occur. What answer best explains this?
a. The mechanism of DNA and RNA phosphodiester bond formation is similar
b.. It is still an adenine containing nucleotide
c. DNA nucleotides are structurally distinct from RNA nucleotides
d. The ribosomes cannot utilize an mRNA molecule that has DNA nucleotides.
3. You want to design a protein that interacts with DNA and cleaves it specifically. Which would the best protein system to use?
a. CRISPR case9
b. Zinc finger nuclease
c. A restriction enzyme
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