12 Jun Chelex beads are added to a solution containing cells
Question
Practice Questions.
Fill in the appropriate words that best complete the blanks below:
1. ……….is the field of science that identifies biological information in DNA
using computer-based tools.
2. Nucleotide sequence are amplified by ————— and the amplified
product is called…………….
3. The locus for Tas2R38 gene is on chromosome number……………
4. The recognition sequence for HaeIII restriction enzyme is………………
5. A laboratory method used to separate DNA based on their sizes………………
6. An enzyme that can be found in a lyophilized pcr bead?
7. ……………..is used as a reference to determine the size of a nucleotide
sequence during gel-electrophoresis
8. A PTC strong taster having a genotype TT will have how many bands when
DNA sample is analyzed on a gel………….
9. Apparatus used to view stained bands of DNA in a gel
10. The ability taste of monosodium glutamate is called…………….
Write the following acronyms in full:
11.SNP………………….
12.BLAST………………
13.PCR………………….
14.PTC………………….
Indicate whether Yes or No
15.E-value means Error value……..
16.A primer with an E-value of 2 X 10-2 is a good primer…………..
17.Tas38R2 gene are present in the cheek cells of PTC tasters……….
18.Boiling a digested DNA at 370C will help to render the cell membrane
permeable……………
19.The DNA of a moderate taster (Tt) will have double bands on a gel………..
20.If the length of a primer is 221bp, an undigested PCR product would retain
the same length……..
21.dNTPs and Taq polymerase can be found in chelex beads………… (found in
PCR beads)
22.Chelex beads are added to a solution containing cells to remove cations?
23.Boiling cheek cells in a thermal cycler makes the cell membrane more
permeable?
24.After running PCR amplification process, the final products could be stored
in an incubator (70oC) in order to preserve the DNA?
25.Forward primer alone is sufficient to isolate a nucleotide sequence of
interest?
26. A DNA runs from the negative electrode towards the positive electrode…..
Answer true or false in the following questions
27.Distilled water is preferred to pure tap water when de-staining during
electrophoresis……….
28.When pipetting a volume of 15µl, a micropipette ranging from 200-1000µl is
preferred to 10-50µl?
29.EcoR1 is preferred to HaeIII as a restriction enzyme in PTC tasting
experiment……………..
30.Ethylidium bromide is preferred to Carolina blue stain because it is noncarcinogenic……….
In the following scenarios, indicate whether the cheek cells can be found ABOVE,
BELOW or DISPERSED.
31.Rinse mouth with saline + pour in a cup + vortexing?
32.Mouth rinsed with saline solution + pour in a cup + centrifugation?
33.Chelex beads + saline solution containing cheek cells + centrifugation?
34. Mouth rinsed with saline solution + pour in a cup + centrifugation +
Resuspension
35.Mouth rinsed with saline solution + pour in a cup + Resuspension +
centrifugation
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