13 Jun AGRY 32000 List three genetic processes that are considered
Answer the following questions. Due Thursday, June 23 at the beginning of class.
1. List three genetic processes that are considered as chromatin remodeling.
2. In Figure 8-23, is it the virus gene or the endogenous gene being repressed and why?
3. The illustration directly below shows a portion of a gene that is being transcribed, with the
template and coding strands labeled, and nucleotide sequences provided. a) Draw an RNA
polymerase as it nears the end of transcription of the DNA sequence; b) indicate the direction
the RNA polymerase is moving; c) write sequence and indicate polarity of the RNA transcript
from the DNA given; d) indicate where the promoter for this gene would be located.
4. Diagram the nucleotide sequences of the a) dsDNA and b) mRNA transcript that both encode
the following amino acid sequence of a polypeptide: Trp-Leu-Cys-Met. c) Indicate polarity.
d) Why is this exercise the reverse of the Central Dogma of Molecular Biology?
2016 AGRY 320, Genetics, Homework 2 40 Points
NAME:
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5. a) Draw a three-intron eukaryotic gene and its pre-mRNA and mRNA products. Be sure to
include the b) promoter, c) conserved sequences in the promoter and their position, d) the
transcription initiation site, e) the untranslated regions, and the f) labeled 5’ to 3’ ends, g)
plus the processing events required to produce the mRNA.
2016 AGRY 320, Genetics, Homework 2 40 Points
NAME:
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6. In the DNA replication fork diagrammed below, the original strands are shown in 2 dimensions,
not as a spiral. a) Indicate the direction of the replication fork, b) the template strands, c) the
leading and lagging strand, and their direction of synthesis. d) Using arrows to indicate
direction of synthesis, draw the new strands that will be synthesized, their polarity, and use
the letter “P” to show the RNA primers.
7. Imagine you genotype two yeast lines and find that they possess functional, wild-type alleles at
for every gene in the Gal pathway, with the exception that one lines possesses null mutant
alleles for GAL3, and the other possess null mutant alleles in GAL80. You grow the two yeast
lines on selective growing media that differ by presence or absence of galactose. This
experiment is represented in the table below. Indicate whether the Gal pathway will be off, on,
or activated by providing these four answers in each corresponding blank box.
Galactose composition in growth media.
Yeast line genotype.
gal3
GAL80
GAL3
gal80
galactose present
galactose absent
2016 AGRY 320, Genetics, Homework 2 40 Points
NAME:
8. The following list contains statements that apply to replication, transcription, translation, some
or all of those things, or are not applicable. Indicate in the box which apply.
Involves the ribosome
The new strand is made 5’ to 3’.
Steps are initiation, elongation, termination
Both DNA strands serve as a template
Exons are removed
Synthesis of the new strand is initiated at the origin
DNA ? protein
The process is done only during the S-phase of the cell cycle.
The new strand is identical to the template strand, with the exception of
U’s replacing T’s.
The template strand is RNA.
The new strand is complementary to the template strand.
Occurs in the cytoplasm
The new strand is made 3’ to 5’.
DNA synthesis
Nucleotide sequence is read from a fixed starting point
Occurs in the nucleus
The product is DNA.
Introns are removed
Synthesis of the new strand is initiated at a promoter.
RNA ? protein
The product is RNA.
Relies on complementary base pairing
Both DNA strands serve as the coding sequence
One DNA strand is the template, while the other is the coding sequence
protein ? RNA
Both DNA strands serve as the template sequence
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