13 Jun A gene expression company is working on a viral infection site in the brain
Question
You have been a life saver for me. Can you help with the following questions please. Thank you so very much in advance for all your help.
Q1. You have been hired by a plant biotechnology company. The first task assigned to you is to isolate a gene that is expressed ONLY in the roots of a soybean plant. Describe in detail one approach, listing all the steps involved, that you could use to isolate gene X (this is a single gene)?
Q2. A gene expression company is working on a viral infection site in the brain of monkeys to identify gene expression changes in that area. To do that they need a pure sample of infected cells from that site and then perform analysis on that. They also want to get the control sample from the same animal.
Clearly delineate a method that can be used for isolation of a pure population of infected cells from that brain site
Where/Which area will you isolate the control cells from
How will you identify genes that are specifically expressed in the viral infected area
Q3. You are working for a Biotech company that has developed a proprietary protein expression system in prokaryotes. They are however, having a lot of trouble with the stability of proteins. What can they do to make the proteins stable in the expression system that they have and also easy to isolate?
Q4. Let’s say you have two tubes, 1 and 2, each carrying a bacterial sample. In one of the tubes the bacteria has been transformed with ONLY a vector and in the tube with a RECOMBINANT (vector +insert) construct. Using ONLY two techniques-PCR and gel electrophoresis (use both), how will you determine which tube carries a recombinant and which tube the vector alone?
Describe where the primers will anneal (not specific sequence but general location), what you expect to observe on the gel and how you will analyze and draw conclusions from the results? A schematic may be helpful!
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.