14 Jun MCB X110 – The function of the normal gene
Question
You have been working hard in an immunology research group to create “knock-out” mice with homozygous deletions in the following genes: 1) Rag 1 & Rag 2 (double mutant); and 2) CD8. Somehow, your mutant strains have been mixed up and now you must perform tests to determine which strain is which. For each of the two mutant strains listed, briefly describe:
i. The function of the normal gene.
ii. The expected mutant phenotype. (Be specific. Explain the cellular/physiological phenotype, and NOT simply that the mouse is sick.)
iii. The specific immunological tests you would perform to distinguish between them. (Describe two different tests. Hint, choose from tests described in module 3, topic 3.4) Be sure to explain what particular reagents you would use, the expected results, and how these results will help you make the correct determination.
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