15 Jun Electrospray ionization mass spectrometry (ESI-MS) of a cytochrome
Question
Name three types of laboratory treatments that can be used to denature a protein.
Briefly describe experimental evidence which supports the view that primary structure determines conformation of a protein.
Complete the following table with the missing terms. (8 points)
Monomers
Bond Type
Biological Macromolecule
amino acids
phosphodiester
polysaccharidesQuestion
Name three types of laboratory treatments that can be used to denature a protein.
Briefly describe experimental evidence which supports the view that primary structure determines conformation of a protein.
Complete the following table with the missing terms. (8 points)
Monomers
Bond Type
Biological Macromolecule
amino acids
Question
Electrospray ionization mass spectrometry (ESI-MS) of a cytochrome c yielded two successive (adjacent) peaks with m/z values of 1365.76 (p1) and 1516.40 (p2). Calculate the mass of this cytochrome c based on these data. Remember that the measured m/z values of two adjacent peaks (p1 and p2) arise from ions with charges z1 and z1-1 (p1 and p2 differ by a single proton with p2 having one fewer positive charge due to its higher m/z value): (1) p1 = (M + z1)/z1 and (2) p2 = (M + z1 – 1)/(z1 – 1)
phosphodiester
polysaccharides
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