15 Jun BIS 103 BIS 103 – Your weight is __________ lbs which is equal to
Question
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NPB101L Section 10 – Quiz 6 Take Home
The “rules of engagement” for the take-home quiz:
You may use any written references you deem necessary (e.g. lab manual, textbooks) but you MAY NOT consult with other students (past or present,) other TAs, professors, neighbors, relatives, pets, extraterrestrials, friends (real or imaginary), beings from another plane of existence, etc. Please make a copy of your quiz to assist you during the lab exercise.
For each question, you must show all your work, include appropriate units, and box your answer to receive full credit.
1. Your weight is __________ lbs which is equal to ________ kilograms. The lab manual (p. 67) specifies how much you should drink based on your weight. Calculate the four volumes (you only need to show the details of the calculations for one of the experimental solutions): (2 pts)
a.) The volume of distilled H2O you should drink: __________
b.) The volume of isotonic saline you should drink: __________
c.) The volume of bicarbonate you should drink: __________
d.) The volume of fluid you should drink if you are the control: __________
2. You remember that you last urinated at 10:40 AM. It is now 1:15 PM. You completely void your bladder, filling a one-liter bottle. What is your flow rate [express your answer in mL/min]? (1 pt)
3. Now assume that your flow rate is 2.75 mL/min. Estimate the creatinine concentration of your urine. You will need to use the estimates of GFR and plasma creatinine concentration given in the lab manual (p. 70). Note: Express your answer in mg/L (1 pt).
4. In order to accurately measure creatinine concentration using the spectrophotometer, you must dilute your estimated creatinine concentration to 30 mg/L. Using the value of estimated creatinine concentration from question 3, calculate the required dilution factor in order to wind up with a urine creatinine concentration of 30 mg/L?. Note: Round your answer UP to the next whole number (2 pt)
5. Assuming you start with a 1 mL sample of urine, how much distilled water would you have to add in order to obtain a dilution factor of 12 (2 pt)?
6. Suppose your optical density (O.D.) reading from the photospectrometer is 1.1. Assume the standard curve to estimate the creatinine concentration of your DILUTED urine sample results in the following equation (this will be provided by the TA):
Calculate the creatinine concentration of your DILUTED urine sample using the equation above.
Using the dilution factor calculated in question 4, find the creatinine concentration of your UNDILUTED urine. Note: Express your answer in mEq/L (1 pt)
7. Using the undiluted urine creatinine concentration from question 6 and the flow rate from question 2, calculate your creatinine clearance (2 pt).
8. Is the creatinine clearance rate that you just calculated close to the expected normal value for a healthy adult? If not, (approximately; include units) what should it be (1 pt)?
9. Suppose your sodium meter reading is 80 mV. Using the standard curve (see last page), calculate your sodium clearance using the flow rate from question 2. (1 pt)
10. When should you prepare your diluted urine samples for creatinine analysis (1 pt)?
11. Which of the following is applicable for this lab (1 pt)?
a. Cleanliness counts! Points will be deducted for messy bathrooms and/or workstations.
b. There will be one door for entering the lab, and one door for leaving the lab.
c. All dilutions will be reacted with picrate at the same time.
d. Urine will only be discarded in the bathroom toilets.
e. Jen will be a stickler this week on cleanliness (both bathroom and workstations), following the protocol, lab etiquette (including no gloved hands on door handles, computers, people, etc), and other things she may think of before Tuesday.
f. All of the above!
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