16 Jun What types of fiber autoradiography patterns would this produce
Question
1) Suppose that DNA replication is unidirectional. What types of fiber autoradiography patterns would this produce?
2) What is the energy source for DNA replication?
3) Could Meselson & Stahl have used radioactive labeling in their experiments?
4) If a mistake is made during replication and not corrected by proofreading, how does the machinery distinguish the ‘good’ strand from the ‘mutant’ strand?
5) How could you use a Northern blot to show that alternative splicing was occurring?
6) Why might the expression of a gene be regulated by multiple TFs?
7) Why do most TFs bind to the major groove of DNA?
8) How might I use a gene chip to identify the targets of a TF?
9) How do transcription factors bind to specific cis-acting sequences?
10) How did operons evolve? Do eukaryotes have them? Why or why not?
11) How do histones interact with DNA?
12) Can histones from one species package DNA of another species?
13) What is the experimental evidence that histones are highly conserved?
14) What effect would a mutation in a nuclear localization signal have on cell function
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