25 Jun The restriction digest map of pUC19 is shown in Figure
Question
The restriction digest map of pUC19 is shown in Figure 4. Suppose you performed restriction
digestions of pUC19 by the various enzymes listed in Table 1 (each row represents a separate
digestion),
1) predict the sizes of fragments in base pairs and write your answers in Table 1;
2) Label the positions of the wells, label the lanes with the enzymes used. Include a lane with
500 bp DNA ladder ( Fig 2). Draw the corresponding DNA fragments on the gel diagram,
and label the size of each DNA fragment. Label the cathode and anode.
0
EcoR I 396
Ava II 2059
pUC19 2686 bp
Bsey I 1110
Ava II 1837
Figure 4. Restriction map of pUC 19, a 2,686 base pair plasmid. The
number after each restrictionenzyme name indicates at which base pair the
DNA is cut by that enzyme.
Enzymes
EcoRI
Bsey I
Ava II
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