25 Jun Mitosis & Meiosis Following chromosomes DNA Movement
The title is Lab 11: Mitosis & Meiosis Following chromosomes DNA Movement.
This lab includes:
*1/Experiment 2: Mitosis Beads Diagram (needs to be filled in).
* 3 Post-Lab questions (needs to be answered)
All the information is available via attachment
Materials
2 Sets of Different Colored Pop-it® Beads (32 of
each – these may be any color)
(4) 5-Holed Pop-it® Beads (used as centromeres)
Procedure:
Mitosis
Genetic content is replicated during interphase. DNA exists as loose molecular strands called chromatin; it has
not condensed to form chromosomes yet.
Sister chromatids begin coiling into chromosomes during prophase. Begin your experiment here:
1. Build a pair of replicated, homologous chromosomes. 10 beads should be used to create each
individual sister chromatid (20 beads per chromosome pair). The five-holed bead represents the
centromere. To do this…
a. Start with 20 red beads to create your first sister
chromatid pair. Five beads must be snapped together for
each of the four different strands. Two strands create
the first chromatid, and two strands create the second
chromatid.
b. Place the five-holed bead flat on a work surface with the
node positioned up. Then, snap each of the four strands
into the bead to create an "X" shaped pair of sister
chromosomes.
c. Repeat this process using 20 new beads (of a different
color) to create the second sister chromatid pair. See
Figure 6 for reference.
Figure 6: Bead set-up. The blue beads
represent one pair of sister chromatids
and the red beads represent a second
pair of sister chromatids.
2. Assemble a second pair of replicated sister chromatids; this
time using 12 beads, instead of 20, per pair (six beads per each complete sister chromatid strand). Snap
each of the four pieces into a new five-holed bead to complete the set up.
© 2013 eScience Labs, LLC.
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3. Repeat this process using 12 new beads (of a different color) to create the second set of sister
chromatids. See Figure 7 for reference.
4. Configure the chromosomes as they would appear in each of the stages of the cell cycle (prophase,
metaphase, anaphase, telophase, and cytokinesis). Diagram the images for each stage in the section
titled "Cell Cycle Division: Mitosis Beads Diagram". Be sure to indicate the number of chromosomes
present in each cell for each phase.
Cell Cycle Division: Mitosis Beads Diagram:
Prophase
Metaphase
Anaphase
Figure 7: Second set of replicated
chromosomes.
Telophase
Cytokinesis
1. Why are chromosomes important? What information do they provide?
2. How many chromosomes did each of your daughter cells contain?
3. How often do human skin cells divide? Why might that be? Compare this rate to how frequently
human neurons divide. What do you notice?
© 2013 eScience Labs, LLC.
All Rights Reserved
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