25 Jun CHANGE THAT OCCURRED IN THE INJECTED RATS
Question
Endocrine Assignment
The data for this assignment were compiled from seven sets of male laboratory rats,
two rats per set; one set was the control group and the remaining six were experimental
groups. The rats were all male to simplify the study of the relationship between the
reproductive and endocrine systems. In each set of rats there was an ‘‘intact’’ rat and a
‘‘castrate’’ rat. The castration involved removal of the testes to eliminate testosterone
production. The two rats (normal and castrate) of each group were treated alike in all
other ways (food, water, etc.). All rats, except for those in the control group were
injected with a hormone on a daily basis for 2 wk. Autopsies were performed on the
animals at that time.
The group of students performing this exercise were very disorganized and rushed
through the work, making errors in labeling the bottles of hormone. The students
obtained the following results for organ weights after the autopsies were performed. In
this short period of time, the students noted amazing changes in the size of certain
organs when they compared the experimental group of rats with the control group.
Using the autopsy data, determine the identities of the unknown hormones.
To help in determining the identity of the unknown hormones, look for changes between
the control values and the values of the unknown hormone (both the intact and castrate
animal). The changes between the control rats and the rats that were treated with the
unknown hormone should be at least 20% if they are to be considered significantly
different. If the change is less than 20%, it is attributed to experimental or biological
error. Experimental errors may include small errors in calibration procedures,
measurements, or instrumentation. Any variability that occurs because of the differences
between animals is considered biological error.
Figure 1 represents the organs of the rats used in the experiment. Figure 2 shows your
set of control rats; the next six figures show the autopsy data from each of the six
experimental groups.
FIG. 1
Graphic representation of organs studied in the autopsy.
1
FIG. 2
Autopsy results from control rats.
2
3
4
5
6
7
8
Answer Sheet
Name(s):
What was the identity of Hormone 4?
Briefly explain every change that occurred in the injected rats.
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