25 Jun How many fragments will result if you linearize the above ds DNA
Question
rviswanathan,
I unlocked one of your documents and this is one of the questions i had a question about it. If a double-stranded circular DNA molecule has 4 recognition sites for HindIII, how many fragments will be generated if you digest that molecule with HindIII? How many fragments will result if you linearize the above ds DNA molecule and then digest with HindIII? Explain
the answer that you had was–>
A ds circular DNA with 4 sites for a restriction enzyme will generate four fragments because one of the sites will linearise it. A linear DNA will give 5 fragments.
–> Can you explain it in detail as to how you obtained it? If you can give a step by step how you got the fragments and linear DNA fragments? and the other question i had was this one–If you are tasked with screening a genomic library for a specific DNA sequence and a cDNA expression library for the expression of a specific protein, what kind of screening methods will you use for each and why? the answer you provided for this one was –> A cDNA library can be screened for a particular protein using the protein fragment complementation assay (PCA) technique using Green Fluorescent Protein (GFP) as a reporter. Interactions of a ‘bait’ protein of interest and ‘prey’ cDNA fragment brings the fragments of the “reporter” protein in close enough proximity to allow them to form a functional reporter protein whose activity can be measured…How did you get this answer? I have looked all over the book and notes and have yet to get that. Please help. any advice or feedback would be greatly appreciated.
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