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Explain what is meant by “indicator” organisms when testing

Explain what is meant by “indicator” organisms when testing

Question
By Week 10, Day 3, consider the following questions: assignment 1

Explain what is meant by “indicator” organisms when testing a water sample. What are the media used to determine the presence of the indicator organisms? What are the morphological and biochemical characteristics of these indicator organisms?
Discuss the three major species of Staphylococcus. Explain why one of them is more pathogenic compared to the other two species. Which factor determines the pathogenesis of the organisms?
On the same day, present your responses to both the questions in a one-to-two page Word document formatted in APA style and submit it

BIO2071_Microbiology Laboratory

Review Sheet Week 10

Exercise 11

Questions

1. In what areas of the hospital must the numbers of microorganisms in the

environment be strictly reduced to the minimum?

2. Why do microbiologists wear laboratory coats? Did you confirm that this is

necessary?

3. Why is it necessary to wear clean, protective clothing when caring for a

patient?

4. Why should hair be kept clean and out of the way when caring for

patients?

5. How can the number of microorganisms in the environment be controlled?

6. When and why is hand washing important in patient care?

7. How can those who care for patients avoid spreading microorganisms

among them?

Exercise 20.1 and 20.2

Questions

1. Differentiate the microscopic morphology of staphylococci and

streptococci as seen by Gram stain.

2. What are the two types of staphylococcal coagulase?

3. What is protein A? Describe one method of detecting it.

4. What properties ofS. aureusdistinguish it fromS. epidermidisandS.

saprophyticus?

5. How isS. saprophyticusdistinguished fromS. epidermidis?

6. From what specimen type wouldS. saprophyticusmost likely be isolated?

7. What is a nosocomial infection? Who acquires it? Why?

8. Why are staphylococcal infections frequent among hospital patients?

9. Describe the role played byS. aureusin human infectious diseases.

Page 1 of 2

FromLaboratory Manual & Workbook in Microbiology Applications to Patient Care (9th ed.). By

Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato Copyright © 2006 The McGraw-

Hill Companies, Inc. Reprinted with permission of The McGraw-Hill Companies, Inc.

BIO2071_Microbiology Laboratory

Exercise 34

Questions

1. What is the bacteriologic standard for potable water?

2. Why is bacteriologic analysis of water directed at recognition of coliforms

and enterococci rather than isolation of pathogenic bacteria?

3. Define presumptive, confirmed, and completed tests of water.

4. Why must positive presumptive tests of water be confirmed?

5. In the Most Probable Number table, what is the significance of the 95%

confidence limits in relation to the MPN index/100 ml?

6. What is the public health significance of coliform-contaminated water?

7. List at least three waterborne infectious diseases.

Page

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