What components must be added to a PCR reaction in order to synthesize DNA

26 Jun What components must be added to a PCR reaction in order to synthesize DNA

Question
QUESTION 1

1. What components must be added to a PCR reaction in order to synthesize DNA? Select all that apply.

dNTPs

ATP

Mg2+

sigma factor

DnaA

primase

helicase

primers

template DNA

QUESTION 2

1. Which strand of DNA is in the RNA polymerase active site during transcription?

coding strand

template strand

both

neither

QUESTION 3

1. Eukaryotes have 3 RNA Polymerases that transcribe unique sets of genes. What genomic feature confers specificity and determines whether a gene is transcribed by RNA Pol I, II, or III?

promoter

enhancer

start codon

+1 nucleotide

polyA sequence

introns

coding region

QUESTION 4

1. What type of RNA is made by the RNA Pol II? Select all that apply.

mRNA

tRNA

rRNA

QUESTION 5

1. What types of gene(s) have polyadenylation cleavage signals (AAUAAA) downstream of the transcription start site? Select all that apply.

tRNAs

rRNAs

snRNAs

mRNAs

siRNAs

QUESTION 6

1. Which of the following are required for mRNA splicing by the spliceosome? Select all that apply.

endonuclease

snoRNA

microRNA

tRNA

ligase

snRNA

QUESTION 7

1. Which of the following modifications are made to tRNAs? Select all that apply.

base modification

5′ methyl G cap

intron splicing

addition of polyA nucleotides

addition of polyT nucleotides

addition of CCA

QUESTION 8

1. Select the one answer that shows the correct order of sequence elements in a bacterial gene.

i

ii

iii

iv

v

QUESTION 9

1. The trp operon of E. coli contains five genes (trpE, trpD, trpC, trpBand trpA) for the production of five proteins. The trp operon: Select all that apply.

contains 1 promoter

contains 5 promoters

encodes 1 mRNA

encodes 5 mRNAs

encodes 1 translational start site

encodes 5 translational start sites

QUESTION 10

1. Trp Repressor (TrpR) regulates expression of the trp operon. The product of one of the genes in the trp operson is indole glycerophosphate synthetase, an enzyme required for tryptophan synthesis. Based on the function of the genes in the operon, what is the likely effect of tryptophan binding to TrpR?

increased binding of TrpR to DNA upstream of the transcription start site

increased binding of TrpR to DNA downstream of the transcription start site

decreased binding of TrpR to DNA upstream of the transcription start site

decreased binding of TrpR to DNA downstream of the transcription start site