26 Jun Alcohol dehydrogenase (AD) is an enzyme
Question
Alcohol dehydrogenase (AD) is an enzyme which catalyzes the reaction of its natural substrate ethanol to form acetaldehyde.The Km of AD, from rhinoceros livers, for ethanol is 1 X 10-3M. This enzyme is however somewhat non-specific and will recognize substrates other than ethanol. How would the kinetic plot be affected if AD were to separately catalyzemethanol and isopropanol instead of ethanol?Assume that the overall Vmax remains the same in all 3 cases.How would the Km change for methanol compared to ethanol (higher, lower, the same)?How would the Km change for isopropanol compared to ethanol (higher, lower, the same)?How would the Km’s of methanol and isopropanol compare (which higher than the other or about the same).Based upon your knowledge of the mechanisms by which enzymes work,briefly explain how you decided to place your new Km’s.
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