26 Jun BIOL3111L SeparationandDetection ofProteinsPre-Work
Question
BIOL3111L
SeparationandDetection ofProteinsPre-Work
1. The clear protein ofan egg white becomes opaque and firm when cooked.
Using your knowledge ofmacromolecules explain what happened ata molecular level.
2. Proteins have various functions within a cell. Describe the characteristics ofprotein structures thatresult in thisdiversity. Be specific.
3. Explain the purpose ofthe following materials forthe protein separation lab:
a.SDS
b.DTT
c.heat
4. What is “osmotic shock” and why it is used in this lab?
5. Which arrow points toa peptide bond?
(fromhttp://www.biology.arizona.edu/biochemistry/problem_sets/large_molecules/01Q.htmlas of11/17/08)
.0/msohtmlclip1/01/clip_image002.gif”>A. 1
B. 2
C. 2 and 3
D. 3
E. 4 and 5
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