26 Jun : Molecular Biology
Question
Glucagon cDNA
(Points: 10)
Go to the NCBI database and find the cDNA for human glucagon. Access code NM_002054. (keep the page open as there will be several questions).
How many nucleotides are described in this deposited sequence.
1. 1293
2. 1294
3. 1295
4. 2054
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2.glucagon introns
(Points: 10)
How many introns are reported for human glucagon?
(access cod NM_002054.4)
1. none
2. one
3. five
4. six
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3.Glucagon exon 1
(Points: 10)
How many amino acids are coded by exon 1 ?
1. none
2. 3
3. 12
4. 24
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4.glucagon homology
(Points: 10)
In which animal is the glucagon sequence 84% similar to human
1. Dog
2. Guinea Pig
3. Chimpanzee
4. Degu
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5.Size of intron 1
(Points: 10)
What is the size (in nucleotides) of the first intron. Hint: you will not get the answer from this page but you may be able to find it in NC_000002.11
1. 247
2. 3298
3. 2971
4. 3318
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6.Alignment
(Points: 10)
Align the glucagon sequences for human, cow (NM_173916.3) and chicken(Y07539.1).
Find the conserved region that includes SRRAQDFVQWLM. Immediately after this what is asparagine substituted for in the chicken?
1. glutamate
2. serine
3. glycine
4. proline
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7.genome size
(Points: 10)
Which of the following animals has the largest genome size
1. zebrafish
2. drosophila
3. duckweed
4. c. elegans
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8.amino acid classes
(Points: 10)
Which of these amino acids can be classified in the same class
1. Gly, Arg, Lys
2. Glu, Asp, Val
3. Gly, Ala, Ile
4. Pro, Glu, Met
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9.Histones
(Points: 10)
How many polypeptides assemble togeter to form a nucleosome?
1. 2
2. 4
3. 6
4. 8
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10.DNA methylation
(Points: 10)
What chemical group is added to DNA in the process of methylation?
1. Fe2+
2. Mg2+
3. CH3
4. COOH
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