nucleic acid polymerases Column preview DNA polymerase II RNA Primase DNA RNA polymerase

26 Jun nucleic acid polymerases Column preview DNA polymerase II RNA Primase DNA RNA polymerase

Question
Polymerases

(Points: 10)

In Eukaryotes what is the product of the following nucleic acid polymerases

Column preview

DNA polymerase II

RNA

Primase

DNA

RNA polymerase I

mRNA

RNA polymerase II

large rRNA

RNA polymerase III

tRNA

Matching pairs

DNA polymerase II

Primase

RNA polymerase I

RNA polymerase II

RNA polymerase III

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2.Cofactor RNA polymersase

(Points: 10)

Which of the following ions is required by RNA polymerase?

1. Ca++

2. Mg++

3. Cl-

4. Fe+++

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3.Promoter clearance

(Points: 10)

Which of the following definitions best describes the process of ‘promoter clearance?

1. It is when an inhibitor is cleared from the promotor to allow RNA polymerase to bind

2. It is when RNA polymerase has transcribed the first few nucleotides and moves away from the promotor regions and transcribes the rest of the gene.

3. It is when the chromatin moves away from the promoter to allow the RNA polymerse to bind

4. It is when DNA methylaton of a promoter region clears it from any DNA binding proteins

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4.Phosphorylation of RNA pol

(Points: 10)

Which of the followings are roles of the phosphorylaton of C terminal Domain of RNA polymerase?

1. It increases the speed of transcription

2. It increase the specificity of the transcription process

3. It pauses transcription to allow for capping to occur

4. It facilitates termination of transcription

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5.Helix turn helix

(Points: 10)

Which class of proteins have a important functional domain termed ‘helix-turn-helix’

1. DNA polymersaes

2. RNA polymerses

3. Helicases

4. transcription factors

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6.Gal4 UAS T/F

(Points: 10)

Gal 4 is a yeast transcription factor that binds to a specific DNA sequence referred to as UAS and it promotes RNA synthesis

True
False

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7.Alternative splicing

(Points: 10)

Which of the following may not be consequences of RNA splicing

1. Insertion of amino acids in the protein sequence

2. Deletion of amino acids in the protein sequence

3. Substitution of amino acids in the protein sequence

4. Formation of chimeric proteins

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8.Capping

(Points: 10)

Which nucleotide is added in the process of RNA capping ?

1. ATP

2. CTP

3. GTP

4. TTP

5. none of the above

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9.RNA editing

(Points: 10)

What are the potential outcome of RNA editing in terms of protein sequence?

1. Single amino acid substitution

2. Single amino acid insertion

3. Single amino acid deletion

4. Early termination

5. none of the above

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10.polyAdenylation

(Points: 10)

Polyadenylation of mRNAs occurs in both prokaryotes and Eukaryotes and it has the same function

True
False

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