27 Jun Hands-On Labs, Inc. Version 42-0056-00-01 Lab Report Assistant -Hands,Photosynthesis & Respiration ,CellMembrane
Question
Plant Genetics
Hands-On Labs, Inc.
Version42-0104-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables that shouldbeaddressedinaformallabreport. Theintentistofacilitatestudents’writing oflab reports by providing this informationin an editable filewhich can be sent to an instructor.
.gif”>Observations
Data Table 1: Punnettsquare.
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Data Table 2: Seedling data.
Seedling Color
Green
Yellow
Total
Numberof
Seedlings
Percentageof
Total
Data Table 3: Frequency calculations.
Frequency
Expected
Values
Actual Values
Green
Yellow
Total
Data Table 4: Corn kernel data.
Number of Kernels
Kernel Percentage
(Divide count by total, then
multiplyby 100)
Kernel Coloration
Purple
Yellow
Total
KernelTexture
Smooth
Wrinkled
Total
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Data Table 5: Punnet square for dihybrid cross.
.jpg”>
Data Table 6: Data from corn kernels.
Number Counted
Ratio: Number counted / total
Purple and smooth
Purple and wrinkled
Yellowand smooth
Yellowand wrinkled
TOTAL
Exercise 1: Observing a Monohybrid Cross
Questions
A. What are the predicted ratiosof the phenotypes in the plants?
B. What was the total number of seeds that germinated?
C. What are the actual ratiosof the phenotypes displayed in the tobacco plants? D. Explain why it may be important to collect data from a larger population.
E. Ifallyellowseedlings wereremovedfromthepopulation,wouldthenextgenerationstillhave a chance at displaying the yellow allele? Explain.
F. Wouldacrossbetweenahomozygousandaheterozygousparentshowthesameratios?
Explain.
Exercise 2: Dihybrid Crossing with Corn
Questions
A. What are the probable phenotypes of the corn ear parents with regard to coloration?
B. What are the probable texture phenotypes of the corn parents?
.8181819915771px;”=””>Photosynthesis & Respiration
Hands-OnLabs,Inc.
Version 42-0102-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables that shouldbeaddressedinaformallabreport. Theintentistofacilitatestudents’writing oflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Observations
Data Table 1: PhotosyntheticRates
Rate
Distance (mm)
Solutionstarting measurement
Net photosynthesis
Respiration
Gross photosynthesis (net photosynthesis + respiration)
Rate of photosynthesis (gross photosynthesis / 10 min)
Bag mass g + 1.5 g NaHCO3 = Final mass (g)
Data Table 2: Respiration Results
Experimental
Conditions
Startingdistance
(mm)
Final distance
(mm)
Change in movement
(mm)
Time
(min)
WithElodea
WithoutElodea
Questions
Exercise 1:PhotosynthesisPartI
A. What molecules did the plant produce when exposed to light? B. What resources were consumed to produce the molecule?
C. What part did light play in the reaction?
D. What molecule was consumed by the plant while it was in the dark? E. What molecule was produced by the plant while it was in the dark? F. Where did the molecules go that were produced in the dark?
G. What criticalservice do plants provide humans?
H. Would animal life on Earth be possible without plants?
I. Would any life on Earth be possible without sunlight?
Exercise 2:PhotosynthesisPartII
A. When youblewthroughthestrawintotheBromothymolbluesolution,whatgasdidyouadd? What color change did you observe in the solutionand why?
B. Whatcolorchangeoccurredafteryouplacedtheplantintotherespirometerandturnedon the light?
C. What was happening when the solutionin the horizontal tubing started to move? D. How long did it take the color change to occur afterthe solutionstarted to move? E. Why did the color change?
F. When you repeated the experiment without a plant in there spiro meter,what happened and why?
CellMembraneTransport
Hands-OnLabs,Inc.Version42-0034-00-01
Lab Report AssistantThisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables that shouldbeaddressedinaformallabreport. Theintentistofacilitatestudents’writing oflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Exercise1:DiffusionthroughanArtificialMembrane
Observations
Data Table 1: Changes During Dialysis
Color
Before Dialysis
AfterDialysis
Solutionin cup 1
Solutionin dialysis tubing
Data Table 2: Benedict’s Reagent Results
Color of Solution
Before Heating
AfterHeating
IKI solutionin cup 1
Solutionin dialysis bag in cup 2
Distilled water
Questions
A. What is the purpose of this exercise? What is being tested?
B. What color change did you observe in the dialysis tubing? What does that change indicate? C. Was there a color change in the water surrounding the tubing? If so, explain.
D. WhatcolorchangedidyouobserveinthewatercontainingtheIKIafterheating?Whatdoes that change indicate?
E. What does the Benedict’s reagent detect? F. What does the IKI solutiondetect?
G. In what way is a cell membrane similar to the model cell made of dialysis tubing? H. Is the transport mechanism in the model cell passive or active?Why?
Exercise 2: Diffusionat differenttemperatures
Observations
Data Table 3: Diffusionof KMnO4at Various Temperatures and Times
Temperature inside cup
Colorof Water
0min
5min
Questions
I. How does temperature affectthe rate of diffusion?
J. State a general hypothesis to cover how temperature affectsrate of diffusion
Exercise 3: Tonicity and Diffusion
Observations
Data Table 4: Potato Dimensions.
Potato
BeforeOsmosis
(L× W) cm
AfterOsmosis
(L× W) cm
Tonicity
Hypertonic, Isotonic, or
Hypotonic
Distilled water
10% Sodium Chloride
Questions
A. Whatistheconditionofeachpotatostripaftersoakinginthetesttubesforanhour?Which one is limp and which one is crisp?
B. Howwouldyou explainthedifferenceintheconditionsofthepotatostripsusingtheconcept of tonicity?
C. What was the tonicity of the fresh water solutionwith respect to the potato cells? D. What was the tonicity of the salt water solutionwith respect to the potato cells?
E. How do the changes in the conditions of the potato strips relate to the wiltingof plants?
F. How does keeping vegetables cool slow them from wilting?
Mitosis
Hands-OnLabs,Inc. Version42-0091-00-01
Lab Report AssistantThisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables that shouldbeaddressedinaformallabreport. Theintentistofacilitatestudents’writing oflab reports by providing this informationin an editable filewhich can be sent to an instructor.
MitosisWorksheet-Answer the questionsabout the photos below:
Onion root tipphotos at 600x and 1000x taken by Stan Carpenter of Hands-On Labs, Inc.
.jpg”>
.jpg”>1. What phase is this cell undergoing?
2. What happens afterthis phase?
1. The two center side-by-side cells are undergoingtwodifferent phasesof mitosis.
a. What are these phases?
b. Whatdiagnosticfeaturescanhelp you with your decision?
.jpg”>
.jpg”>1. What phase is this cell undergoing?
2. How can you tell?
1. This cell has clearly visible chromatids.
a. What’sthenextphasethiscellwill undergo?
b. Wherewillthechromatidsgointhe coming phase?
.jpg”>
.jpg”>1. Thecellinthelowercenterisexhibiting signs of what phase of mitosis?
2. Whatphasedoesitappearthecellaboveis undergoing?
1. Explainwhathappens duringanaphase.
2. When thechromatidsseparate,whatis each called?
Questions
A. What is the purpose of mitosis?
B. Why is the cell cycle important for organisms?
C. What role does mitosis play in the growth of an embryo?
D. What is one main differencein the mitoticprocesses between plant and animal cells?
E. Ifthecellthathasundergonemitosisoriginallyhadthediploid (2n)numberofchromosomes, then what number of chromosomes will the nuclei in the two new cells have?
F. Can mitosis ever be used for reproduction?
G. What parts of plants are most impacted by mitosis?
H. Why might mitosis be easier to study in the tipof the root?
I. Where else in a plant might you finda large amount of mitoticcell division occurring? J. How might the mitoticindex be applied in agriculture?
K. Whatdidyoucomputetobethemitoticindexofthecombinedsixslides(assumethereare
54totalcells)?Incomparison totheindexesreflectedinFigure8,fromapproximatelywhicharea
of a root was the sample taken?
.8181819915771px;”=””>Extractionof DNA
Hands-On Labs, Inc. Version 42-0056-00-01
Lab Report Assistant
Thisdocumentisnotmeanttobeasubstitute foraformallaboratoryreport.TheLabReport Assistantissimplyasummaryoftheexperiment’squestions,diagramsifneeded,anddatatables that shouldbeaddressedinaformallabreport. Theintentistofacilitatestudents’writing oflab reports by providing this informationin an editable filewhich can be sent to an instructor.
Questions
A. Describe what the DNA from the split peas looked like?
B. Wereyourexperimentalresultswhatyouexpectedtosee?Specifically,wastheremoreor less DNA than you expected?
C. Whatwasthepurposeofaddingthedetergenttotheexperiment?Ifthedetergentwasnot used, do you think the experiment results would have changed? Explain your answer.
D. Ifyouused5%isopropylalcoholinsteadof91%isopropylalcohol,doyouthinktheexperiment results would have changed? Explain your reasoning.
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