27 Jun BIO – Pre-Lab Questions (Lab 3)
Question
Pre-Lab 3 Questions Name___________________
Section _________
Score ___/8
Read over the lab before coming in to class and answer these questions. Hand them in to the instructor at the beginning of lab.
Assign the termphenotype orgenotype to the following:
red-
heterozygous-
dominant-
green-
What is the purpose of making up a Punnett square?
Why don’t the flies we’ll use for this lab have wings?
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Pre-Lab Questions: Evolutionary Connections lab
In an evolutionary tree, what does the trunk represent?
.95pt; font-size: 11px;”>What two types of molecules would a molecular biologist study to determine evolutionary relationships
.95pt; font-size: 11px;”>Why is it more useful to do a comparison of 3 or more organisms than just two?
.95pt; font-size: 11px;”>What will our control organism be in the experiment today?
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Using Indirect Measurements
Evolutionary Relationships between Domesticated Animals
Procedure
1. There is a culture dish filled with an agar gel. In this gel you will poke seven holes in a circular pattern with one hole in the middle of the circle. As seen in figure 1 and figure 2.
Figure 1.
2. After poking the holes, on the bottom of the dish write next to each hole the following.
1) Anti for Cow serum antibodies
2) D for donkey serum
3) G for goat serum
4) C for cow serum
5) H for horse serum
6) S for sheep serum
7) C for chicken serum
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3. Once you’ve completed labeling, pipet 15 uL of the appropriate substance into the proper well.
4. Next week we will exam the gels and come up with conclusions. The plates will resemble figure 2.
Figure 2.
Questions
1. Compare the serum proteins from the various mammals in terms of immunological properties. Do you think these properties are a reliable index for studying evolutionary relationships? Explain your answer.
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2. Describe how your results would have been affected if the antiserum was made in rabbits against duck serum proteins.
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3. Why is it important to include a cow serum sample along with the other species?
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