30 Jun Look at the attachment below and complete the pamplet questions.
BIOS 213 Lab Week 10 Today’s y assignments g Complete: Lab 22 Antibiotic Sensitivity Case Study #1 Staphylococci and Micrococci Begin: Case Study #2 Enterics and Pseudomonads Your unknown will be oxidase negative and the following tests will not be available: Arginine Dehydrolase, O-F Glucose, and Triple Sugar Iron. Choose topic for Pamphlet BIOS 213 Lab Week 10 Finish Lab 22 Antibiotic Sensitivity Measure diameter of zones Results: Only y2&3 All discussion BIOS 213 Lab Week 10 Case Study #1 Staphylococci and Micrococci Flowchart Results Table Questions 2-5 Carbohydrate Test BIOS 213 Lab Week 10 Case Study #2 Enterics and Pseudomonads Your unknown will be oxidase negative and the following tests will not be available: Arginine g Dehydrolase, y , O-F Glucose,, and Triple Sugar Iron. Please show me the flow chart you have prepared. BIOS 213 Lab Week 10 Enterics – Phylogeny Domain: Bacteria Kingdom: Eubacteria b Phylum: Proteobacteria Cl Class: Gammaproteobacteria Order: E t Enterobacteriales b t i l Family: Enterobacteriaceae Gram (-) rods Ferment D-Glucose Most are catalase (+) All are oxidase (-) BIOS 213 Lab L b Week W k 10 Pathogenic Enterics Salmonella enteritis salmonellosis Shigella enteritis s ge os s shigellosis Yersinia pestis Bubonic plague Escherichia coli Travelers diarrhea BIOS 213 Lab L b Week W k 10 Pseudomonads – Phylogeny Domain: Bacteria Kingdom: Eubacteria b Phylum: Proteobacteria Cl Class: Gamma Proteobacteria Order: Pseudomonadales Family: Pseudomonadaceae Gram (-) rods Aerobic Al Almost always l motile il Most are catalase (-) Most are oxidase (+) BIOS 213 Lab Week 10 Pseudomonas aeruginosa •Opportunistic pathogen isolated from wounds, abscesses, burns and ear infections •Produces an exotoxin A which is secreted into the host tissue Example of Flow chart ENTEROCOCCI AND STREPTOCOCCI WEEK 11-CASE STUDY #3 10 POINTS You will not be able to use the Hemolysis, Hippurate or Litmus Milk tests Introduction • Streptococcus genus is divided into 6 groups: – Pyogenic streptococci – Oral streptococci – Enterococci – Lactic streptococci – Anaerobic streptococci – Other streptococci • Typically cells in these groups are spherical or ovoid which occurs in pairs or in chain • These are gram positive bacteria Results on Blood Agar • The main characteristic within ithi thi this group off bacteria is the ability/inability to hemolyze y blood. • Hemolysin: an enzyme that lyses red blood cells. • β β-Hemolytic: y complete p lysing of red blood cells. • α-Hemolytic: green zone around colonies due to the loss of potassium in red blood cells. • γ-Hemolytic: no reaction α-, β- and γ-hemolysis on sheep blood agar Pyogenic bacteria • completely breaks down red blood cells and usually has polysaccharide l h d antigens in their h cell ll wall. ll • Lancefield groups- different polysaccharide antigens are divided into different serological groups depending upon their h i composition. ii S Streptococcus pyogenesLancefield A and S. agalactiae- Lancefield D • Most are β-hemolytic. • Most bacteria found in the pyogenic streptococci are pathogenic to both human and animals • Causes otitis media,, bacteremia,, pneumonia, p , meningitis g and endocarditis Oral Streptococci • Gram positive, non motile cocci but some may be grow as short rod like S. mutans • Mostly found in oral cavity Fecal streptococci • Includes bacteria from two g genera: Enterococcus and Streptococcus • Enterococcus faecalis is indicator of human fecal g origin • Other includes E. faecium, E. avium, E. gallinarum, S. bovis, S. equinus, S. mitis and S. salivarus • Lancefield group D bacteria except S. mitis and S. salivarus (Viridans group) Enterococcus – causes nosocomial infection – Spec Species es from o this t s genus ge us make a e up 12% % of o all a nosocomial infection – E. faecalis and E. faecium – bacteremia, endocarditis and some cases meningitis Pamphlet – 30 points due 4-24-19 Format: size 10/12, work cited attached, typed, proper grammar Treatment/Prevention (3 each) Effort/Creativity 3 pts 6 pts 3 pts I t Intro: Facts/ Statistics History Characteristics of the organism g that causes the STD 4 pts 3 pts 5 pts t How do you know that you have the STD? Symptoms Tests 3 pts 3 pts BIOS 213 Lab Week 10 Lab 22 Antibiotic Sensitivity Results: Only 2 & 3 All discussion Lab Report for Case Study #2 Enterics and Pseudomonads Flowchart R Results lt T Table bl Questions 2-5 Prepare flowchart P fl h t ffor Case Study #3 Enterococci and Streptococci Pick a topic for your pamphlet …
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