23 Jul WHAT DOES THE YELLOW FOOD COLORING REPRESENT AT THE END OF THE EXPERIMENT?
In the kidney filtration Experiment. What does the yellow food coloring represent at the end of the Show more In the kidney filtration Experiment. What does the yellow food coloring represent at the end of the experiment? What does the Congo Red respresent? Experiment 1: Kidney Filtration The kidneys function to filter the blood in the body by waste removal. In this experiment the dialysis bag represents a part of the kidney. The solution containing the Congo Red yellow food coloring and water symbolizes blood as it enters the kidney through the renal artery. As the experiment progresses notice the filtration occurring with the kidney (dialysis tubing) and the resulting substances. Procedure 1. Begin by placing the dialysis tubing in a beaker filled with 200 mL water. Submerge the tubing for 10 minutes. 2. Remove the tubing from the water after 10 minutes. Use your forefinger and thumb to rub the sides of the dialysis tubing apart. This will create a tube-like shape. Refill the beaker to 200 mL if the volume has decreased. 3. Secure a small rubber band around the bottom of the dialysis tubing to seal it. Wrap the rubber band around the dialysis tubing as many times as possible. Test that the dialysis tubing will not leak out of the bottom by placing a few drops of water into the tubing. If water leaks out the bottom the rubber band has not been fastened tight enough. If water does not leak pour the water out of the tubing into the sink. Set the tubing aside. 4. Grab one 250 mL beaker and fill it with 200 mL of water. Set this aside for now. 5. Use the 10 mL graduated cylinder to measure 3 mL of Congo Red. Pour this into the empty 250 mL beaker. Rinse the graduated cylinder. 6. Use the 10 mL graduated cylinder to measure 3 mL of yellow food coloring. Pour this into the 250 mL beaker with the Congo Red. Rinse the graduated cylinder. 7. Use the 10 mL graduated cylinder to measure 5 mL of water. Pour this into the 250 mL beaker with the Congo Red and yellow food coloring. Materials 30 cm Dialysis Tubing 2 Small Rubber Bands Pipette 3 mL Congo Red 3 mL Yellow Food Coloring (2) 250 mL Beakers 10 mL Graduated Cylinder *Water *You must provide 475 The Urinary System 8. Take a pipette and mix the solutions in the 250 mL beaker. To do this place the pipette in the solution and squeeze and release the bulb of the pipette while moving the pipette throughout the solution. 9. Pipette 10 mL of the mixed solution into the dialysis tubing and complete Table 1 by indicating which solutions are present in each container. 10. When all 10 mL have been placed into the dialysis tubing seal the top of the dialysis tubing by wrapping place a second rubber band around the top of the dialysis tubing. 11. Place the sealed dialysis tubing into the 250 mL beaker with 200 mL of water. 12. Wait 60 minutes. Look for any diffusion that may have occurred through the dialysis tubing (inbound or outbound). Indicate in Table 2 which solutions are present in each container. P Show less
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