23 Jul FOR WHICH PEPTIDE SHOULD YOU MAKE A BUFFER?
H2N-Arg-Val-Thr-Ala-Asp-CO2H and H2N-His-V Show more Suppose you have a mixture of the following two peptides. H2N-Arg-Val-Thr-Ala-Asp-CO2H and H2N-His-Val-Thr-Ala-Asn-CO2H Peptides and amino acids are least soluble when they are zwitterions (at their isoelectric point pI). In order to separate the mixture of peptides you wish to make up a buffer at pH = pI of one of the peptides in order to selectively precipitate it from solution. a.) Draw each peptide structure b) Calculate the pI of each peptide using the sidechain pKa(s) of the individual amino acids (neglect local functional group effects) c.) Buffers work better near the pKa of one of the buffering components. For which peptide should you make a buffer? Show less
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