23 Jul DETERMINE KM AND VMAX USING THE LINEWEAVER-BURKE PLOT.
Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as b-lactamase) Show more Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as b-lactamase) an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kD. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-9 g of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay. a. Plot V0 versus [S] for these data. Does penicillinase appear to obey Michaelis- Menten kinetics? If so what is the value of Km? b. What is the value of Vmax? Indicate on the plot how you determined Km and Vmax. c.Write the Michaelis-Menten equation and show the derivation of the Lineweaver-Burke equation. d.Plot 1/V0 versus 1/[S]. e. Determine Km and Vmax using the Lineweaver-Burke Plot. Indicate on the plot how you determined Km and Vmax. Show less
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