23 Jul WHY DO THESE TWO DNA SAMPLES GIVE DIFFERENT RESULTS WHEN THEYRE BOTH 50% G-C?
DNA into 1000 bp fr Show more E. coli DNA and hippopotamus DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation you will find that all the DNA bands at the same place in the gradient and if you graph the distribution of DNA fragments in the gradient you will get a single peak. If you perform the same experiment with hippopotamus DNA you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to two small satellite peaks on a graph of the distribution of DNA fragments in the gradient. Why do these two DNA samples give different results when theyre both 50% G-C? Show less
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