23 Jul WHAT IS THE ORDER OF THE LACZ AND LACY GENES RELATIVE TO PROC?
In E. coli the ability to utilize lactose as a carbon source requires the presence of the enzymes B Show more In E. coli the ability to utilize lactose as a carbon source requires the presence of the enzymes B-galactosidase and B-galactoside permease. These enzymes are encoded by two closely linked genes lacZ and lacY respectively. Another gene proC controls in part the ability of E. coli cells to synthesize the amino acid proline. The alleles str^r and str^s respectively control resistance and sensitivity to streptomycin. Hfr H is known to transfer the two lac genes proC and str in that order during conjugation. A cross was made between Hfr H of genotype lacZ- lacY+ proC+ strs and an F- strain of genotype lacZ+ lacY- proC- strr. After about 2 hours the mixture was diluted and plated out on medium containing streptomycin but no proline. When the resulting proC+ strr recombinant colonies were checked for their ability to grow on medium containing lactose as the sole carbon source very few of them were capable of fermenting lactose. When the reciprocal cross (Hfr H lacZ+ lacY- proC+ strs X F- lacZ- lacY+ proC- strr) was done many of the proC+ strr recombinants were able to grow on medium containing lactose as the sole carbon source. What is the order of the lacZ and lacY genes relative to proC? Im really struggling to understand why this is. Ive drawn so many pictures but I still dont understand. Show less
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