24 Jul WHAT ARE THE UNITS (ABBREVIATED) FOR THE INDEPENDENT VARIABLE?
Isolated rat liver cells were placed in a dish with b Show more Interpret the Data How the Experiment Was Done Isolated rat liver cells were placed in a dish with buffer at physiological conditions (pH 7.4 37C). Glucose 6-phosphate (the substrate) was added to the dish where it was taken up by the cells. Then a sample of buffer was removed every 5 minutes and the concentration of ~P i determined. Data from the Experiment Time (min) Concentration of ~P i (?mol/mL) 0 0 5 10 10 90 15 180 20 270 25 330 30 355 35 355 40 355 1. To see patterns in the data from a time-course experiment like this it is helpful to graph the data. First determine which set of data goes on each axis. (a) What did the researchers intentionally vary in the experiment? This is the independent variable which goes on the x-axis. (b) What are the units (abbreviated) for the independent variable? Explain in words what the abbreviation stands for. (c) What was measured by the researchers? This is the dependent variable which goes on the y-axis. (d) What does the units abbreviation stand for? Label each axis including the units. 2. Next youll want to mark off the axes with just enough evenly spaced tick marks to accommodate the full set of data. Determine the range of data values for each axis. (a) What is the largest value to go on the x-axis? What is a reasonable spacing for the tick marks and what should be the highest one? (b) What is the largest value to go on the y-axis? What is a reasonable spacing for the tick marks and what should be the highest one? 3. Plot the data points on your graph. Match each x-value with its partner y-value and place a point on the graph at that coordinate. Draw a line that connects the points. 4. Examine your graph and look for patterns in the data. (a) Does the concentration of ~P i increase evenly through the course of the experiment? To answer this question describe the pattern you see in the graph. (b) What part of the graph shows the highest rate of enzyme activity? Consider that the rate of enzyme activity is related to the slope of the line ?y/?x (the rise over the run) in ?mol/mL ? min with the steepest slope indicating the highest rate of enzyme activity. Calculate the rate of enzyme activity (slope) where the graph is steepest. (c) Can you think of a biological explanation for the pattern you see? 5. If your blood sugar level is low from skipping lunch what reaction (discussed in this exercise) will occur in your liver cells? Write out the reaction and put the name of the enzyme over the reaction arrow. How will this reaction affect your blood sugar level? Show less
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