25 Jul DOES THE INHIBITOR Z BEHAVE AS A COMPETITIVE OR NON-COMPETITIVE INHIBITOR?
. There are simple assays that ut Show more Consider a GTPase that catalyzes the reaction: GTP + H2O GDP + Pi. There are simple assays that utilize a color reaction to measure the amount of Pi in a solution. Suppose you utilize such an assay to measure the velocity of the release of Pi catalyzed by the GTPase. In addition you have an inhibitor Z that inhibits the GTPase. You vary the concentration of GTP and measure the product released in the presence and absence of an inhibitor and obtain the following data: mmol Pi released/min mmol Pi released/min [GTP] mM no inhibitor +100 M inhibitor Z 180.00000 1.00000 0.74627 200.00000 1.00000 0.81301 240.00000 1.25000 0.93458 330.00000 1.55000 1.16279 500.00000 1.82000 1.51515 1000.00000 2.90000 2.12766 1) Using these data what is the Vmax of the uninhibited GTPase? ______ mmol/min 2) Using these data what is the Km of the uninhibited GTPase? ______mM 3) Does the inhibitor Z behave as a competitive or non-competitive inhibitor? a) Competitive inhibitor b) Non-competitive inhibitor Show less
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