25 Jul DO YOU EXPECT M TO BE POSITIVE OR NEGATIVE?
Proteins can be unfolded or denatured by addition of certain small molecules such as urea. Such sp Show more Proteins can be unfolded or denatured by addition of certain small molecules such as urea. Such species are called denaturants and we will denote the molar concentration of added denaturant as cD. A protein can be in a properly folded state F or in an unfolded state U. Under standard conditions and in the absence of any added denaturant (cD = 0) the F state is more stable and it has a lower standard free energy. The standard free energy difference between F and U is given by ?G 0 (0) = G 0 U G 0 F > 0 where ?G 0 (0) in units of energy/mole is the standard free energy change at zero concentration of denaturant (as indicated by the zero in parentheses). As denaturant is added at concentration cD the standard free energy difference between F and U changes to a new value ?G 0 (cD). a.) A denaturant works by affecting the free energy of F and of U differently. A simple model known as the linear energy model assumes that the standard free energy difference between F and U as a function of concentration of D is: (3) where m is a constant. Recalling that we are working with standard free energies having units of energy/mole what are the units of m? Do you expect m to be positive or negative? Why? Show less
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