29 Jul WHAT ARE THE GENOTYPES FOR EACH PARENT?
Lab Exercise Lesson 2: Multigenic Mendelian Genetics and Testing Mendel’s Hypotheses
Lab Preparation
Textbook: Read Chapter 3 (Principles of Genetics, Snustad & Simmons 2012)
Video: Watch Professor Anderson’s Explanation of Punnett Squares (some topics of are important for this week and some are important for future weeks)
Video: Watch Professor Anderson’s Chi-Square Video
Mendel’s Hypotheses
The results from Medel’s experiments lead to hypotheses which other data from genetic crosses could be tested. In this lab, you will identify what the Mendelian hypotheses for monohybrid and dihybrid crosses are. Then you will use these hypotheses to test whether experimental data are consistent with a hypothesis. You will use a chi-square goodness-of-fit test statistic to accept or reject the hypothesis.
Monohybrid Cross- One Trait
Preform the following cross and determine the Mendelian phenotypic and genotypic ratio. Start with two true breeding parents one dominant (G) and one recessive (g) for the color of peas trait in peas. The dominant allele give a green color and the recessive phenotype is yellow. True breeding means there is very little variation in phenotype from one generation to the next and the trait is likely homozygous. This is the parental generation (P). The parental cross produced an F1 generation. The F1 generation offspring were self-fertilized to produce an F2 generation. A Punnett square is a good way to organize your data.
1. What are the genotypes for each parent?
2. What is the genotype and phenotype for the F1 generation offspring (fill in shaded part of table)?
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