26 Jun You are reading culture plates today and identifying patho
You are reading culture plates today and identifying pathogens that grow from patient samples. Which two pieces of information are absolutely critical in determining which additional tests must be done toidentify the pathogen? Appearance on charcoal agar EndosporeStain Oxygen sensitivity Gram Stain 2 points Question 2 A10year old has a wound on the arm that the physician suspects is infected. Upon cultureyou see small white colonies growing on blood agar as well as chocolate agar. You gram stain the colonies to find that they are gram positive cocci. Which test will you perform first? catalase lactose fermentation coagulase indole 1 points Question 3 You have isolatedcatalasepositive gram positive cocci from a wound culture. Which test will you perform next? A disccontaining bacitracin coagulase P disccontaining optichin 1 points Question 4 You are working on a sputum culture. You seemucoidalpha hemolytic colonies that number many more than the normal flora present. The gram stain of the colonies shows gram positive cocci that are in lancet shaped pairs. Which test will you do? acid fast staining motility P disccontaining optichin A disccontaining bacitracin 1 points Question 5 You are working up a throat culture. Standard procedure in plating throat cultures in your lab includes dropping an A disc near the initialinoculumin the firstquandrantwhen streaking the specimen. You examine the blood agar plate and see moderate normal flora and many beta hemolytic colonies that do not grow up to the A disc. The beta hemolytic colonies arecatalasenegative gram positive cocci. Which pathogen is in this throat culture? Streptococcus agalactiae Streptococcus pyogenes Staphylococcus epidermidis Staphylococcus aureus 1 points Question 6 You are working up a male genital culture. You see no growth on the blood agar plate but small colonies growing on the chocolate agar plate. The gram stain shows gram negative cocci in pairs. Which test will you do next? oxidase motility catalase indole 1 points Question 7 You are working up acerebrospinalfluid culture. You find colonies growing on blood agar as well as chocolate agar. The colonies areoxidasepositive gram negative cocci. The colonies ferment glucose and maltose but not sucrose or lactose. You identify the pathogen as Haemophilusinfluenzae Neisseriagonorrhoeae Streptococcus pneumoniae Neisseriameningitidis 1 points Question 8 You are working up a urine culture. You see >100 colonies that are gray and flat on the blood agar plate and >100 colonies that are bright pink on theMacConkeyagar. TheIMViCresults areIndolepositive Methyl Red positive Vogues-Proskauernegative Citrate negative. You have identified the pathogen as Citrobacterfreundii Enterobacteraerogenes Proteus vulgaris Escherichia coli 1 points Question 9 You are working up a stool culture. OnMacConkeyagar you see many bright pink colonies and many clear colonies. Which colonies are potential pathogens that require further testing? Clear coloniesnon lactose fermenters Clear colonieslactose fermenters Bright pink coloniesnon lactose fermenters Bright pink colonieslactose fermenters 1 points Question 10 DNA technology is useful in the identification of pathogens that are unable to be grown readily on artificial lab media. pathogens that are no longer alive in the patient sample species that cannot be differentiated by conventional testing. All of the above. 1 points Question 11 You are preparing a sample of DNA from an unknown colony of bacteria. After adding digestion buffer and incubating for the time suggested by the manufacturer you centrifuge the sample. The DNA is found stuck to the sides of the tube. stuck to the gel in the tube. in the supernatant in the tube. in the pellet in the bottom of the tube. 1 points Question 12 Which of the following is not true of thePolymerase Chain Reaction? PCR is facilitated by a heat labile DNA polymerase. PCRcan facilitate the detection of DNA that is too low to detect by other methods. PCRis a method of replicating DNA in a test tube. 1 points Question 13 Why aredATPdCTPdTTPanddGTPadded to a PCR reaction tube? Theybuffer the mixture. They catalyze the polymerase. They provide the building blocks of DNA. They allow the DNA in the sample to anneal. 1 points Question 14 Why are universal 16SrDNAprimers used in your experiment? They will anneal to highly conserved areas of the gene that encodes bacterial16SrRNA. They will anneal to unique sequences of genes encoding 16SrRNAin specific bacteria. 1 points Question 15 If universal primers are used to amplify DNA in a PCR reactionthen the PCR product must be sequenced to determine the bacteria that the DNA belongs to. True False 1 points Question 16 How is the PCR product separated from the PCR mixture at the completion of the reaction? Perform electrophoresis in anagarosegel stain the gel and cut the band corresponding to the PCR product from the gel. Pour the PCR mixture into a commercially prepared DNAmicroconcentratorcolumn and follow the manufacturer’s directions to adhere and elute the PCR product from the column. Both of the above procedures may be used. Neither of the above procedures may be used. 1 points Question 17 Your PCR product was sequenced by a method known as Cycle Sequencing. Which of the following statements is false. Tagged terminator nucleotidesfacilitate thecreation ofa seriesofnested DNA sequences of different length. Cycle sequencing can be completed in just one test tube. An automatic sequencer performs electrophoresis and reads the tagged DNA piecesproviding a read out of thenucleotide bases comprising the DNA sequence of the fragment being tested. Cycle sequencing is done in a PCR machine. 1 points Question 18 The National Library of Medicine has adatabankcalledGenBankthat has deposited in it the DNA sequences ofnumerous genes isolated from known bacterial species. True False 1 points Question 19 You obtained the following BLAST data from your sample: 99.9%Enterobactersakazakii 95.2%Enterobacteraerogenes 93.7%Enterobactercloacae The pathogen in your sample is: Enterobactersakazakii Enterobacteraerogenes Enterobactercloacae Enterobacterspecies
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