25 Jun A membrane prep was assayed for NET (NE transporter) binding
Question
A membrane prep was assayed for NET (NE transporter) binding using increasing concentrations of 3H-Nisoxetine in triplicate.
Background binding for each reaction was identified in duplicate using 10 mM desipramine
mg protein
Sample tube# DPM total bckgrd specific per Rxn dpm/mg fmol/mg
1 1 837
2 1329
3 924 0.364
4 189
5 168
2 6 1530
7 1398
8 1737 0.306
9 234
10 249
3 11 5061
12 4668 0.377
13 3660
14 867
15 753
4 16 7470
17 7527
18 7824 0.325
19 771
20 873
5 21 9552
22 8763 0.436
23 8547
24 1290
25 1242
Please answer the following questions:
What is the average total dpm for each reaction?
What is the average “background” dpm for each reaction?
What is the average specific dpm for each reaction?
What is the specific dpm/mg for each reaction?
calculate the fmol/mg of receptor for each reaction, using the following information:
Specific Activity of the 3H-Nisoxetine= 87.2 Ci/mmol
There are 2.2 x 10^6 dpm/mCi [Hint: make the units cancel]
Show your work for converting dpm/mg to fmol/mg – you can get partial credit even if the answer is wrong
Graph the resulting fmol/mg (you can use excel or another graphing program). What is your ballpark estimate of the Bmax?
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.