30 Jun Agarose Gel Electrophoresis for the Separation of DNA Fragments lab report, biology homework help
Wymeshia Robinson SCORE Gel Electrophoresis 97% COACH HELP SCORE ADJUSTMENT Pre-lab Briefing 0% 100% Understand the overall process of gel electrophoresis 100% Understand how to assemble the electrophoresis apparatus 100% Know how to operate a micropipet to load the gel 100% Know how to place the negative and positive electrodes 100% Understand how results of gel electrophoresis are read 100% Understand what the samples are composed of 100% Experiment: Gel Electrophoresis 95% Add ethidium bromide 100% Cast an agarose gel + 100% Add tape to casting tray + 100% Pour agarose into tray + 100% Add gel tray to chamber + 100% Add DNA samples and controls to the gel + 100% Zoom in on chamber to add DNA samples + 100% Use pipette tips before trying to pipette + 100% Add DNA samples to the gel using the autopipette + 100% Return to the main view + 100% Run the gel + 100% Put lid on the electrophoresis chamber 100% Start the power source 100% Allow the agar to cool sufficiently before transferring it to the electrophoresis chamber 100% You correctly timed the cooling of the gel, and so your wells were of good depth and strength. Remember to remove the tape from the gel tray after casting the gel 100% By removing the tape, you were able to assure that your gel was surrounded by buffer. Examine the gel in UV light 100% You remembered that the DNA bands must be viewed under uv light in order to see them. Remember to add electrophoresis buffer before loading DNA samples 100% The buffer is important for conducting electric current, and you added an appropriate amount. Assemble the gel tray correctly 100% You prepared the gel tray in the right way to contain the gel. Remember to include controls when loading the gel 100% You remembered to add your controls. This allows you to have a basis for comparison of results. Remember not to use the same pipette tip for different DNA samples 100% You did a good job remembering to change pipet tips to prevent contamination. Set the voltage correctly for running the gel 100% You correctly set the voltage on the power pack, and got good separation of samples. Let the electrophoresis run sufficiently long to separate the DNA bands 100% You let the gel run for an appropriate amount of time. You correctly watched the bromophenol blue dye front to assess how far your samples migrated. Correctly determine the genotypes of the three unknown samples 0% You did not correctly interpret your results. Remember how to use your control to identify which bands are short fragment DNA and which are long. Experimental probes Know the consequence of setting the voltage too low during electrophoresis 100% 100% What happens if you set a low voltage during the electrophoresis? Did you know the answer: You said ‘Unsure’. You answered correctly: The migration of the DNA bands will be slow. Know the consequence of setting the voltage too high during electrophoresis 100% What happens if the voltage is set too high during electrophoresis? Did you know the answer: You said ‘Unsure’. You answered correctly: A high voltage can heat up the buffer and cause the gel to melt. Know the purpose of the plexiglass cover on the UVtable 100% What is the purpose of the plexiglass screen of the table? UVDid you know the answer: You said ‘Unsure’. You answered correctly: The plexiglass screen protects the eyes against radiation. UV- Know how to cast an agarose gel 100% What is the correct order for preparing an agarose gel? Did you know the answer: You said ‘Unsure’. You answered correctly: • While heating the agarose gel mixture, prepare the casting tray by sealing the ends with masking tape and placing the comb to create sample wells. • Add ethidium bromide to the agarose solution. • Pour the agarose solution into the gel casting tray. • Let the agarose solution solidify for at least 10 minutes before use. Post-lab review 100% Know the purpose of gel electrophoresis 100% Know the purpose of the electrophoresis buffer 100% Know how to prepare the gel for electrophoresis 100% Know how to load the DNA samples 100% Know how to set the voltage for electrophoresis 100% Know how to visualize the DNA bands 100% Understand the relationship of voltage to rate of movement of DNA 100% Know the relationship between DNA fragment size and rate of movement 100% Know which sample functions as the control 100% Know what tumor necrosis factor is 100% …
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