25 Jul ANALYZE THE GEL AS A WHOLE USING THE FIRST GEL PICTURE ON THE NEXT PAGE.
Complete the first Show more Gel #1: Analyze the gel as a whole using the first gel picture on the next page. Complete the first column in the table below. Gel #2 Student #4: Analyze the gel as a whole using the second gel picture on the next page. Analyze the quadrant of the gel for the designated student number. Complete the remaining columns in the table below. Answer the following questions about the labeled gel. For the Explanation section put an X in boxes where the option is possible. Leave boxes where the option is not possible blank. Gel number 1 2 2 2 Sample name A-COI A-COI P-rbcL B-16S Number of PCR bands in the lane Approximate sizes of the bands in the lane (bp) Explanation Ethidium bromide was not added to the gel Component of the Taq mix was degraded No primers in the master mix DNA template not added to PCR tube Not enough DNA in genomic DNA prep or bacterial culture Contamination of genomic DNA or PCR sample Non-specific primer binding Samples switched when loaded on the gel PCR was performed correctly Sample should be sequenced Please Help! Show less
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