14 Jun Assignment 2: pH Optimum for Invertase Another factor
Question
Assignment 2: pH Optimum for Invertase
Another factor that strongly influences enzyme activity in living cells is the pH of the environment in which the enzyme is designed to function. For example, in humans, a cytoplasmic protein in a skin cell is surrounded by a different fluid environment at a different pH than a membrane-bound enzyme like invertase with an active site that projects out into the lumen of the small intestine. The following exercise is designed to help you understand the effect of pH on enzyme activity by studying invertase activity over a range of different pH values from acidic to basic conditions.
Formulate a hypothesis to predict the effect of pH on invertase activity.
Set up an experiment at the optimal temperature that you determined in assignment 1, with a substrate concentration of 90 mM. Beginning at the lowest pH value, 3.0, measure invertase activity, find the slope of the line, record data, and repeat this process for other buffer solutions with different pH values. Run experiments for at least two different buffers for each whole number change in pH units until you reach the maximum pH value of 10.0 (e.g., 3.0, 3.4, 4.0, 4.4, 5.0, 5.4).
Create a plot of VO vs. pH. Click on this plot and drag the gray dashed pH line until you find Vmax. When this line is correctly aligned, you will have found the pH optimum for invertase under these reaction conditions. The optimal pH value will appear in the best pH text box.
What is the optimal pH for invertase activity? Do the results of this experiment support or refute your hypothesis? Why or why not? Explain your answers? Why and how do pH changes affect invertase activity?
If you were to carry out these pH experiments at a higher or lower temperature, what effect would this have on the pH optimum for invertase? Formulate a hypothesis and then test your hypothesis. What did you discover? Explain your results.
Our website has a team of professional writers who can help you write any of your homework. They will write your papers from scratch. We also have a team of editors just to make sure all papers are of HIGH QUALITY & PLAGIARISM FREE. To make an Order you only need to click Ask A Question and we will direct you to our Order Page at WriteDemy. Then fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Fill in all the assignment paper details that are required in the order form with the standard information being the page count, deadline, academic level and type of paper. It is advisable to have this information at hand so that you can quickly fill in the necessary information needed in the form for the essay writer to be immediately assigned to your writing project. Make payment for the custom essay order to enable us to assign a suitable writer to your order. Payments are made through Paypal on a secured billing page. Finally, sit back and relax.
About Writedemy
We are a professional paper writing website. If you have searched a question and bumped into our website just know you are in the right place to get help in your coursework. We offer HIGH QUALITY & PLAGIARISM FREE Papers.
How It Works
To make an Order you only need to click on “Order Now” and we will direct you to our Order Page. Fill Our Order Form with all your assignment instructions. Select your deadline and pay for your paper. You will get it few hours before your set deadline.
Are there Discounts?
All new clients are eligible for 20% off in their first Order. Our payment method is safe and secure.