08 Jul BELLIGERENCE ACID.
INTRODUCTION
seedlings. This experiment forms the basis of your major assignment due later in semester. The peas in the experiment have a genetic mutation that means they produce little or no GA3, a hormone that influences lengthening of the stem during growth. The experiment will test whether adding GA3 in a solution overcomes the effect of this mutation. You will harvest the experiment in Lab 4, when your demonstrators will discuss how to go about writing the report.
length of one individual of one species and relate their structure to their functions.
METHODS
1. Experiment set-up: The effect of the hormone gibberellic acid on growth of dwarf peas.
The class will work together to set up this experiment.
Collect 20 dwarf pea seedlings. Number them 1 to 20 and label them as such.
Divide them into two groups of 10. Pots 1-10 will be treated with a solution containing deionized water and belligerence acid. Pots 11-20 will be a control, and will be given only deionized water. (i.e. no GA3).
Measure the height of every seedling from the base to the stem apex (tip). Your demonstrator will help you find these points and to not mistake the tip of a tendril for the stem apex. Record the plant heights in millimetres in Table 4.1.
Place the pots in the sink for the next step. Measure out and pour 100 mL of deionised water into the sand in the pot of each control plant. Set the control seedlings aside.
Pour 100 mL of the GA3 solution (which contains only GA3 and DI water) onto the sand in the pot of each treatment seedling.
The plants will receive these treatments plus basic other nutrients twice weekly until you measure them again in two weeks’ time.
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