11 Jun BIO 353 – Assume to a sample of a purified phospholipid
Question
1. Assume to a sample of a purified phospholipid you have added cholesterol. The
entire sample is in the liquid crystal state. What happens as you progressively drop the
temperature of the sample 1°C an hour until you reach -5°C? Describe the events that
will happen.
2. What difference in results will you observe if you compared the melting point of two
samples? Sample 1 has is made of a single type of phospholipid. Sample 2 has 35
different types of phospholipid in them.
3. List the components of homogenization buffer.
4. Would Tris (Trizma) buffer be a good buffer to be placed in an intracellular buffer?
Explain your answer.
5. Explain the condition of the detergent that was used to isolate the transmembrane
proteins.
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