10 Jun BIOLOGY 5612 – Cell Biology, Microscopes
uestion
Cell Biology: Microscopes
1) If you could use any color in the spectrum of visible light to observe your specimens in the microscope, what color would you use to obtain the highest resolution? Explain.
2) Nmae 3 differences between upright and inverted compound microscopes.
3) In what circumstances would you use an upright, an inverted and a fluorence microscope?
4) Would you use the same filter cube for every fluorophore you use in your experiment?
1) which light source will provide you with greater resolution green, blue or red light?
2) whch objective is most useful to resolve a mitochondria (apprx, size = 750 nm)?
3) how does the image differ in terms of orientation from the unmagnified image?
4) since the depth of field only allows you to focus a limited number of focal planes at a time; will you be able to tell what is above or below in your biological sample? If no, why not? If yes, how?
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